[1]周伟玲,赵启仁.时间分辨荧光分析技术的研究进展及应用[J].国际放射医学核医学杂志,2006,30(2):103-106.
 ZHOU Wei-ling,ZHAO Qi-ren.The development and application of time-resolved fluorescence assay[J].International Journal of Radiation Medicine and Nuclear Medicine,2006,30(2):103-106.
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时间分辨荧光分析技术的研究进展及应用(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
30
期数:
2006年第2期
页码:
103-106
栏目:
体外分析
出版日期:
1900-01-01

文章信息/Info

Title:
The development and application of time-resolved fluorescence assay
作者:
周伟玲 赵启仁
300192 天津, 中国医学科学院、中国协和医科大学放射医学研究所实验核医学室
Author(s):
ZHOU Wei-ling ZHAO Qi-ren
Department of Experimental Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China
关键词:
荧光免疫测定稀土元素荧光共振能量转移
Keywords:
Fluoroimmuno assayLanthanide series elementsFluorescence resonance energy transfer
分类号:
R446.62
摘要:
时间分辨荧光分析(TRFA)技术是一种以镧系元素螫合物为标记物,测量其发射荧光的超灵敏的无放射性污染的标记分析技术,在生物分析中得到了广泛的应用。在近些年,研究发展了酶放大时间分辨荧光分析、时间分辨猝灭分析和分子信标等分析系统,其灵敏度更高,满足了不同的需求,使TRFA技术在临床诊断和科学研究中得到了更广泛的应用。
Abstract:
Time-resolved fluorescence assay is a new nonisotopic and ultrasensitive detection method of using lanthanide chelate as detectable label and detect lanthanide chelate fluorescence, having a great prospect. In recent years, a serial of assay systems have been developed quickly and have more sensitivity, such as enzyme-amplified time-resolved fluorescence assay, time-resolved quenching assay and molecular beacon. They fit for more different requirement, and have been gotten a broader application in clinical diagnosis and science research.

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备注/Memo

备注/Memo:
收稿日期:2005-06-13。
基金项目:天津市自然科学基金资助项目(043610211)
通讯作者:周伟玲(E-mail:zwling@gmail.com)
更新日期/Last Update: 1900-01-01