[1]江波.早熟染色体凝集技术作为生物剂量计的研究[J].国际放射医学核医学杂志,2005,29(6):286-289.
　JIANG Bo.Study on biological dosimetry of premature chromosome condensation technique[J].International Journal of Radiation Medicine and Nuclear Medicine,2005,29(6):286-289.
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
29
期数:
2005年第6期
页码:
286-289
栏目:
放射医学
出版日期:
1900-01-01

文章信息/Info

Title:
Study on biological dosimetry of premature chromosome condensation technique
作者:
江波
300192 天津, 中国医学科学院中国协和医科大学放射医学研究所
Author(s):
JIANG Bo
Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China
关键词:
早熟染色体凝集; 生物剂量
Keywords:
premature chromosome condensation; biological dosimetry
分类号:
Q345+.2;R144.1
摘要:
早熟染色体凝集技术作为生物剂量的测定,利用冈田酸、花萼海绵体诱癌素A孵育非刺激人淋巴细胞诱导早熟染色体凝集取代中期细胞融合的办法,能简便、精确地检测出由辐射诱导的早期损伤。
Abstract:
The premature chromosome condensation technique has been applied for biological dosimetry purpose. Premature chromo-some condensation was induced by incubating unstimulated human peripheral blood lymphocytes in the presence of okadaic acid or calyculin A (a phosphatase inhibitor) which eliminated the need for fusion with mitotic cells. It is now possible to examine the early damage induced by radiation. It is simple, exact when it combines with fluorescence in situ hybridization.

参考文献/References:

1 Iliakis G, Okayasu R, Varlotto J, et al. Hypertonic treatment during premature chromosome condensation allows visualization of interphase chromosome breaks repaired with fast kinetics in irradiated CHO cell[J]. Radiat Res, 1993, 135(3):160-170.
2 Durante M, George K, Wu HL, et al. Rejoining and misrejoining of radiation-induced chromatin breaks Ⅲ Hypertonic treatment[J].Radiat Res, 1998, 149(2):68-74.
3 Kawata T, Ito H, Uno T, et al. G2 chromatid damage and repair kinetics in normal human fibroblast cells exposed to low-or high-LET radiation[J]. Cytogenet Genome Res, 2004, 104(1-4):211-215.
4 Kawata T, Durante M, Furusawa Y, et al. Rejoining of isochromatid breaks induced by heavy ions in G2-phase normal human fibroblasts[J]. Radiat Res, 2001, 156(7):598-602.
5 Durante M, George K, Yang TC. Biological dosimetry by interphase chromosome painting[J]. Radiat Res, 1996, 145(1):53-60.
6 Durante M, Furusawa Y, Majima H, et al. Association between G2-phase block and repair of radiation-induced chromosome fragments in human lymphocytes[J]. Radiat Res, 1999, 151(6):670-676.
7 George K, Durante M, Willingham V, et al. Biological effectiveness of accelerated particles for the induction of chromosome damage measure in metaphase human lymphocytes[J]. Radiat Res, 2003, 160(5):425-435.
8 George K, Wu H, Willingham V, et al. High-and low-LET induced chromosome damage in human lymphocytes:a time-course of aberrations in metaphase and interphase[J]. Int J Radiat Biol, 2001,77(2):175-183.
9 Kanda R, Hayata I, Iloyd DC. Easy biodosimetry for high-dose radiation exposures using drug-induced prematurely condensed chromosomes[J]. Int J Radiat Biol, 1999, 75(4):441-446.
10 Prasanna PGS, Escalada ND, Blakely WF. Induction of premature chromosome condensation by a phosphatase inhibitor and a protein kinase in unstimulated human peripheral blood lymphocytes:a simple and rapid technique to study chromosome aberrations using specific whole-chromosome DNA hybridization probes for biological dosimetry[J]. Mutat Res, 2000, 466(2):131-141.
11 Pantelias GE, Iliakis GE, Sambani CD, et al. Biological dosimetry of absorbed radiation by C-banning of interphsae chromosome in peripheral blood lymphocytes[J]. Int J Radiat Biol, 1993, 63(3):349-354.
12 Turai I, Veress K, Gunalp B, et al. Medical response to radiation incidents and radionuclear threats[J]. BMJ, 2004, 328(7439):568-572.
13 Dainiak N, Waselenko JK, Armitage JO, et al. The hematologist and radiation casualties[J]. Hematology(Am Soc Hematol Educ Program), 2003:473-496.
14 Hayatal I, Kanda R, Minamihisamatsu M, et al. Cytogenetical dose estimation for 3 severely exposed patients in the JCO criticality accident in Tokai-mura[J]. Radiat Res, 2001, 42(3):149-155.

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备注/Memo

备注/Memo:
收稿日期:2005-03-01。
更新日期/Last Update: 1900-01-01