[1]牛津梁.DNA损伤修复能力对GPA基因突变频率的影响及其检测方法[J].国际放射医学核医学杂志,2001,25(6):279-282.
　NIU Jin-liang.The influence of DNA repair ability on GPA mutation frequency and it’s detection[J].International Journal of Radiation Medicine and Nuclear Medicine,2001,25(6):279-282.
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
25
期数:
2001年第6期
页码:
279-282
栏目:
综述
出版日期:
1900-01-01

文章信息/Info

Title:
The influence of DNA repair ability on GPA mutation frequency and it’s detection
作者:
牛津梁
300192 天津, 中国医学科学院中国协和医科大学放射医学研究所
Author(s):
NIU Jin-liang
Institute of Radiation Medicine, Chinese Academy of Medical Science Peking Union Medical College, Tianjin 300192, China
关键词:
血型糖蛋白A; 基因突变频率; DNA损伤; DNA修复; 个体差异
Keywords:
glycophorin A; gene mutation frequency; DNA damage; DNA repair; individual differences
分类号:
Q345+.7
摘要:
血型糖蛋白A(GPA)基因突变频率在作为生物剂量计及癌的风险预测方面最大的缺陷在于个体差异大。DNA损伤及修复能力是影响GPA基因突变频率个体差异的决定因素之一。随着现代分子生物学实验技术的发展,检测DNA损伤及修复能力的方法不断出现,例如中性膜洗脱法、单细胞凝胶电泳、梯度电压凝胶电泳等,使得应用DNA损伤及修复能力对GPA基因突变频率的个体差异进行修正成为可能。
Abstract:
The prominent disadvantage of using GPA mutation frequency as biodosimeter and cancer risk predictor is the significant individual differences. DNA damage and repair ability is one of determinants influencing the individual differences in GPA mutation frequency. With the improvement of current protocols for molecular biology, the measurement of detecting DNA damage and repair ability has been made, such as neutral filter elute, single cell gelelectrophoresis, graded-voltage gel electrophoresis, which make it feasible to modify the individual difference in GPA mutation frequency with DNA damage and repair.

参考文献/References:

[1] Muller WU, Bauch T, Stuben G, et al.Radiation sensitivity of lymphocytes from healthy individuals and cancer patients as measured by the comet assay[J].Radiat Environ Biophys, 2001, 40(1):83-89.
[2] Reitz M, Fritsch G, Lober G, et al.Detection of DNA damage in stimulated human lymphocytes after adding cytostatic drugs in vitro.A model to demonstrate individual damage rates[J].Arzneimittelforschung, 1997, 47(1):88-91.
[3] Kaur BS, Blazek ER.Subdenaturing (pH11.1) filtier elution:more sensitive quantification of DNA doublestrand breaks[J].RadiatiRes, 1997, 147:569-578.
[4] Theodore S.The use of biphasic linear ramped pulsed field gel electrophoresis to quantify DNA damage based on fragment size distribution[J].Int J Radiati Oncol Biol Phys, 1993, 27:659-663.
[5] Dahm Daphi.J Seperation of DNA fragments induced by ionizing irradiation using a graded-field gelelectrophoresis[J].Int J Radiat Biol, 1995, 67(2):161-168.

备注/Memo

备注/Memo:
收稿日期:2001-5-9。
作者简介:牛津梁(1975-),男,天津市人,中国医学科学院中国协和医科大学放射医学研究所硕士研究生,主要从事分子流行病学研究。
更新日期/Last Update: 1900-01-01