[1]谭文庆.单链抗体的研究进展[J].国际放射医学核医学杂志,2001,25(2):55-59.
　TAN Wen-qing.Current situation of the study of single-chain Fv antibody[J].International Journal of Radiation Medicine and Nuclear Medicine,2001,25(2):55-59.
点击复制

单链抗体的研究进展(/HTML)
分享到：

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
25
期数:
2001年第2期
页码:
55-59
栏目:
综述
出版日期:
1900-01-01

文章信息/Info

Title:
Current situation of the study of single-chain Fv antibody
作者:
谭文庆
300192 天津, 中国医学科学院中国协和医科大学放射医学研究所
Author(s):
TAN Wen-qing
Institute of Radiation Medicine, Chinese Academy of Medical Seiences and Peking Union Medical College, Tianjin 300192, China
关键词:
单链抗体; 抗体库; 人源抗体
Keywords:
single-chain Fv antibody; antibody library; human antibody
分类号:
R144.1
摘要:
抗体技术由细胞工程抗体(杂交瘤-单克隆抗体)发展到基因工程抗体,尤其是抗体库技术的出现,将抗体工程发展到了一个新阶段。本文从抗体库技术等方面介绍单链抗体(single-chain Fv antibody,sc Fv)的进展情况。
Abstract:
Antibody technology has been developed from cellular engineering antibody to genic engineering antibody. It has been developing rapidly with the building of antibody library. The current status of single-chain Fv antibody study was summarized.

参考文献/References:

[1] Vaughan TJ,Williams AJ,Pritchard K,et al.Human antibodies with subnanomolar affinities isolated from a large non-immunized phage display library[J].Nature Biotechnol,1996,14:309-314.
[2] Verma R,Boleti E,George AJT.Antibody engineering:Comparison of bacterial,yeast,insect and mammalian expression systems[J].J Immunol Methods,1998,216:165-181.
[3] Krebber A,Bornhauser S,Burnhauser S,et al.Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage system[J].J Immunol Methods,1997,201:35-55.
[4] Vaughan T,Osbourn JK,Tempest PR,et al.Human antibodies by design[J].Nature Biotechnol,1998,16:535-539.
[5] Owen RJ.The genetic engineeringof monoclonal antibodies[J].J Immunol Methods,1994,168:149-165.
[6] Boel E,Verlann S,Poppelier M J,et al.Function human monoclonal antibody of all isotypes constructed from phage display library-denived single-chain Fv antibody fragments[J].J Immurol Method,2000,239:153-166.
[7] Shadidi M,Sioud M.An anti-leukemic single chain Fv antibody selected from a synthetic human phage antibody library[J].Biochem Biophys Res Commun,2001,280(2):649-655.
[8] Griffiths AD,Williams SC,Hsrtley O,et al.Isolation of high affinity human antibodies directly from largesynthetic repertoirea[J].EMBO,1994,13:3245-3260.
[9] Hanes J,Plückthun A.In vitro selection and evolution of functional proteins by using ribosome display[J].Proc Natl Acad Sci USA,1997,94:4937-4942.
[10] Schaffitzel C,Hanes J,Jermutus L,et al.Ribosome display:an in vitro method for election and evolution of antibodies from libraries[J].J Immunol Methods,1999,231:119-135.

相似文献/References:

[1]洪智慧,周小林,石怡珍,等.131I标记抗胃泌素释放前体单链抗体的制备及其稳定性和免疫活性研究[J].国际放射医学核医学杂志,2010,34(2):107.[doi:10.3760/cma.j.issn.1673-4114.2010.02.013]
　HONG Zhi-hui,ZHOU Xiao-lin,SHI Yi-zhen,et al.Preparation and evaluation of 131I-anti-ProGRP(31-98) single-chain antibody fragment[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(2):107.[doi:10.3760/cma.j.issn.1673-4114.2010.02.013]
[2]秦丽莉,张春明.单链抗体研究进展及其在医学中的应用[J].国际放射医学核医学杂志,2005,29(6):255.
　QIN Li-li,ZHANG Chun-ming.The research progress and medicine application of the ScFv antibody[J].International Journal of Radiation Medicine and Nuclear Medicine,2005,29(2):255.

备注/Memo

备注/Memo:
收稿日期:2000/12/5。
作者简介:谭文庆(1970-),男,内蒙古包头人,中国医学科学院中国协和医科大学放射医学研究所硕士研究生,主要从事肌球蛋白单链抗体研究。
更新日期/Last Update: 1900-01-01