[1]龙伟,牟感恩,张广慧,等.黑茶提取物的辐射防护作用及其机制研究[J].国际放射医学核医学杂志,2017,41(6):407-412.[doi:10.3760/cma.j.issn.1673-4114.2017.06.006]
　Long Wei,Mu Ganen,Zhang Guanghui,et al.Anti-radiation effects of dark tea and its mechanism[J].International Journal of Radiation Medicine and Nuclear Medicine,2017,41(6):407-412.[doi:10.3760/cma.j.issn.1673-4114.2017.06.006]
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黑茶提取物的辐射防护作用及其机制研究(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
41
期数:
2017年第6期
页码:
407-412
栏目:
论著
出版日期:
2017-12-05

文章信息/Info

Title:
Anti-radiation effects of dark tea and its mechanism
作者:
龙伟;  牟感恩;  张广慧;  周则卫;  孙元明
300192 天津, 中国医学科学院北京协和医学院放射医学研究所, 天津市放射医学与核医学重点实验室
Author(s):
Long Wei;  Mu Gan’en;  Zhang Guanghui;  Zhou Zewei;  Sun Yuanming
Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China
关键词:
黑茶; 辐射防护; Toll样受体5
Keywords:
Dark tea; Radiation protection; Toll-like receptors 5
DOI:
10.3760/cma.j.issn.1673-4114.2017.06.006
摘要:
目的 研究黑茶提取物抗辐射作用及其作用机制。方法 采用137Cs-γ照射小鼠的30 d存活率实验，受照小鼠肺组织、肝组织和血清的氧化损伤防护试验测定单纯照射组、黑茶提取物（50、100、200 mg/kg）各剂量给药组，以及黑茶提取物1、100 ng/mL对HEK-BlueTM hTLR5细胞中Toll样受体5（TLR5受体）激活实验来探索黑茶提取物的辐射防护作用及其作用机制。结果 30 d存活率实验结果显示，小鼠受到射线7.0 Gy照射后，黑茶提取物（50、100、200 mg/kg）给药组小鼠的平均生存时间从单纯照射组的18.73 d分别提高到23.40、24.07、27.73 d，特别是高剂量组作用明显，差异有统计学意义（t=3.126，P<0.05），黑茶提取物高剂量给药组小鼠的存活率提高幅度达53.4%。氧化损伤防护试验结果显示，黑茶提取物能够提高小鼠血清和组织中超氧化物歧化酶（SOD）（t=2.993，P<0.05；t=4.049，P<0.01）、过氧化氢酶（CAT）（t=4.883，P<0.01）和谷胱甘肽过氧化物酶（GSH-Px）（t=2.702、2.959，均P<0.05）的活性并降低脂质过氧化物丙二醛（MDA）的含量。TLR5受体激活实验结果发现，黑茶主要成分之一的黑茶多糖在低剂量时，与空白对照组相比，能显著激活TLR5受体，差异有统计学意义（t=9.222，P<0.05）。结论 黑茶提取物具有非常显著的辐射防护作用，其作用机制可能与黑茶多糖对TLR5受体的激活有关。
Abstract:
Objective To investigate the anti-radiation mechanism and its mechanism of dark tea extract. Methods The mechanism of radiation protection of dark tea extract groups (50 mg/kg, 100 mg/kg, 200 mg/kg) were investigated by 30 days survival rate experiment of 137Cs γ-rays irradiated mice, protection of oxidative damage in liver, lung and serum, and TLR5 receptor activation test of HEK-BlueTM hTLR5 cell after deal with dark tea extract (1 ng/mL, 100 ng/mL). Results In 30 d survival rate test, average survival time of dark tea extract groups (50 mg/kg, 100 mg/kg, 200 mg/kg) increased from 18.73 d to 23.40 d, 24.07 d and 27.73 d compared with the irradiation-only group, especially in the high dose group (t=3.126,P<0.05). The survival rate of the high dose group was 53.4% lifted. The results of oxidative damage protection test showed that dark tea extract could increase the activity of superoxide dismutase(t=2.993, P<0.05; t=4.049, P<0.01), catalase(t=4.883, P<0.01) and glutathione peroxidase (t=2.702, 2.959, both P<0.05) in serum and tissues of mice, and reduce the content of lipid peroxides malondialdehyde(MDA). The dark tea extract polysaccharide could activate the TLR5 receptor when compared with the control group, the difference was sign that dark tea extract can effectively prolong the survival time of mice from 18.73 days to 27.7 days after irradiated with 7.0 Gy γ-rays, the survival rate of mice treated with dark tea siginificant(t=9.222, P<0.05). Conclusion The data suggested that the radiation protection mechanism of dark tea extract may be related to the activation of TLR5 receptor by dark tea polysaccharide.

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备注/Memo

备注/Memo:
收稿日期:2017-07-07。
基金项目:国家自然科学基金（81673106）；中国医学科学院医学与健康科技创新工程项目（2017-I2M-1-102）
通讯作者:孙元明,Email:sunyuanming@irm-cams.ac.cn
更新日期/Last Update: 2017-12-05