[1]严惟力,孙大裕,林祥通,等.L-[1-13C]苯丙氨酸呼气试验定量检测肝脏功能动物实验研究[J].国际放射医学核医学杂志,2006,30(1):9-13.
　YAN Wei-li,SUN Da-yu,LIN Xiang-tong,et al.Experimental study on L-[1-13C]phenylalanie breath test for quantitative assessment of liver function with animal[J].International Journal of Radiation Medicine and Nuclear Medicine,2006,30(1):9-13.
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L-[1-13C]苯丙氨酸呼气试验定量检测肝脏功能动物实验研究(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
30
期数:
2006年第1期
页码:
9-13
栏目:
实验核医学
出版日期:
1900-01-01

文章信息/Info

Title:
Experimental study on L-[1-13C]phenylalanie breath test for quantitative assessment of liver function with animal
作者:
严惟力1; 2;  孙大裕2;  林祥通1;  蒋义斌2;  孙旭2;  戎兰2;  梁棋2
1. 200040 上海, 复旦大学附属华山医院核医学科;
2. 200040 上海, 复旦大学附属华山医院消化科
Author(s):
YAN Wei-li1; 2;  SUN Da-yu2;  LIN Xiang-tong1;  JIANG Yi-bin2;  SUN Xu2;  RONG Lan2;  LIANG Qi2
1. Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China;
2. Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai 200040, China
关键词:
苯丙氨酸; 呼吸功能试验; 肝脏; 13C排除速率常数
Keywords:
Phenylalanine; Respiratory function tests; Liver; 13C excretion rate constant
分类号:
R817.1
摘要:
目的 采用自行设计的小动物呼气试验模型进行大鼠L-[1-13C]苯丙氨酸呼气试验(13C-PheBT),以验证该实验方法的可行性和有效性,并提供有效的试验参数。方法 280~290g的雄性SD大鼠20只,随机分为急性肝损伤组和正常对照组,每组10只,采用四氯化碳橄榄油灌胃染毒复制急性肝损伤模型,小动物呼气机进行机械通气制作呼气试验模型;按20mg/kg体重尾静脉弹丸给予13C-苯丙氨酸(13C-Phe),收集给药前和给药后1~60min呼出气样共29次气样,应用气体同位素比值质谱仪测定样品中13C丰度。结果 13C排除时相曲线呈单峰,峰值多位于给药后2min;急性肝损伤大鼠呼气试验参数13C排除速率常数(PheBT-K)为(2.45±0.25)×10-2min-1,显著低于正常对照组(2.98±0.19)×10-2min-1(t=5.40,P<0.001),而急性肝损伤大鼠呼出气中13C排除峰值和血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST、总胆汁酸(TBA)、碱性磷酸酶(AKP)和总胆红素(TBIL)含量均显著高于正常对照组(t值分别为8.15,3.40,3.90,4.83和4.12,P<0.05);13C快处置常数在两组间差异无显著性(t=0.58,P>0.05);急性肝损伤大鼠PheBT-K与血清ALT和AKP活度以及TBA和TBIL水平呈负相关(r分别为-0.74、-0.73、-0.82和-0.67,P值均小于0.05),而与血清AST活度无相关性(r=0.16,P>0.05)。结论 自行设计的小动物呼气试验模型是进行呼气试验基础研究的有效工具;动物PheBT-K是一项灵敏的分析指标。
Abstract:
Objective Using a small animal breath test model we designed and L-[1-13C] pheny-lalanine breath test (13C-PheBT) of rats, we investigated its feasibility and validity and determined effective parameter of the test.Methods Twenty male Sprague-Dawley(SD) weighting 280~290g rats randomized into two groups acute hepatitis rats (n=10) and control rats (n=10). Hepatitis was induced by carbon tetrachloride (CCI4)olive oil administration through intragastric gavage. PheBT was assisted by small mechanical ventilator improved and air samples were collected discontinuously, 20mg/kg body weight L-[1-13C] phenylalanine (13C-Phe)was administered intravenously. Twenty-nine breath samples were taken before and different intervals within sixty minutes after administration. 13C enrichment was measured by isotope ratio mass spectrometer.Results All time phase curves of 13C enrichment in rat breath reached a peak almost at 2 min after the intravenous administration of 13C-Phe. The PheBT parameters, 13C excretion rate constant (PheBT-K), of CCI4 hepatitis rats were significantly lower than that of normal control rats[(2.45±0.25)×10-2 min-1 vs (2.98±0.19)×10-2 min-1, t=5.40, P<0.001]. Serum alanine transaminase (ALT), aspartate transaminase (AST), total bile acid (TBA), alkaline phosphatase(AKP) in hepatitis rats were significant higher than that of controls (t value is 8.15, 3.40, 3.90, 4.8 and 4.12 respectively, P<0.05). However 13C fast phase disposition constant did not statis-tically differ between the two groups (t=0.58, P>0.05). PheBT-K had significant negative cor-relation with serum ALT, AKP, TBA and total bilirum TBIL (the correlation coefficient r is-0.74,-0.73,-0.82 and -0.67 respectively, P<0.05), and no statistically significant correlation with serum level of AST(r=0.16, P>0.05).Conclusions It was indicated that the small animal breath test model we designed was a virtual tool to use in experimental study on breath test and PheBT-K was a sensitive index.

参考文献/References:

1 严惟力, 蒋义斌. 肝脏功能呼气试验药物的临床研究进展.国外医学消化系疾病分册,2003,26(6):344-347.
2 严惟力, 蒋义斌. 氨基比林呼气试验.国外医学·放射医学核医学分册,2003,27(5):201-203.
3 Burke PA,Stack JA,Wagner D,et al.L-[1-13C] Phenylalanine oxidation as a measure of hepatocyte functional capacity in end-stage liver disease.Am J Surg,1997,173(4):270-274.
4 Ishii Y,Suzuki S,Kohno T,et al.Patients with severe liver cirrhosis followed up by L-[1-13C] phenylalanine breath test.J Gastroenterol,2003,38(11):1086-1090.
5 孙大裕,严惟力,林祥通,等.L-[1-13C]苯丙氨酸呼气试验定量检测肝功能分析参数研究.中华消化杂志,2003,23(7):399-402.
6 严惟力,孙大裕,林祥通,等.L-[1-13C]苯丙氨酸呼气试验定量检测肝功能研究.核技术,2003,26(5):362-366.
7 Schoonjans R,Van Vlem B,Van Heddeghem N,et al.The 13C-octanoic acid breath test:validation of a new noninvasive method of measuring gastric emptying in rats.Neurogastroenterol Motil,2002,14(3):287-293.
8 Suzuki S,Ishii Y,Asai S,et al.l-[1-13C]Alanine is a useful substance for the evaluation of liver function.J Surg Res,2002,103(1):13-18.
9 Wensing G,Sabra R,Branch RA.Renal and systemic hemodynamics in experimental cirrhosis in rats:relation to hepatic function.Hepatology,1990,12(1):13-19.
10 张克君.肝脏功能障碍量化指标的评价及意义.国外医学外科学分册,2003,30(1):4-7.
11 Tanaka N,Tanaka K,Nagashima Y,et al.Nitric oxide Increases hepatic arterial blood flow in rats with carbon tetrachloride-induced acute hepatic injury.Gastroenterology,1999,117(1):173-180.
12 奚念珠.药物代谢动力学.上海:上海医科大学出版社,1992.

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备注/Memo

备注/Memo:
收稿日期:2005-11-28。
通讯作者:严惟力(E-mail:yan-weili@hotmail.com)
更新日期/Last Update: 1900-01-01