[1]何山震,王淑侠,李石磊,等.高效液相色谱与放射性流动计数器联用确证13N-氨水及测定放射化学纯度的研究[J].国际放射医学核医学杂志,2015,39(3):205-208.[doi:10.3760/cma.j.issn.1673-4114.2015.03.004]
 He Shanzhen,Wang Shuxia,Li Shilei,et al.Identification of 13N-NH3 and analysis of its radiochemical purity using high performance liquid chromatography-ultraviolet-flow-count[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(3):205-208.[doi:10.3760/cma.j.issn.1673-4114.2015.03.004]
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高效液相色谱与放射性流动计数器联用确证13N-氨水及测定放射化学纯度的研究(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
39
期数:
2015年第3期
页码:
205-208
栏目:
出版日期:
2015-05-25

文章信息/Info

Title:
Identification of 13N-NH3 and analysis of its radiochemical purity using high performance liquid chromatography-ultraviolet-flow-count
作者:
何山震 王淑侠 李石磊 王朋
广东省人民医院PET中心(广东省医学科学院), 广州, 510080
Author(s):
He Shanzhen Wang Shuxia Li Shilei Wang Peng
PET Center of Guangdong General Hospital(Guangdong Academy of Medical Sciences), Guangzhou 510080, China
关键词:
色谱法高压液相13N-氨水放射化学纯度
Keywords:
Chromatography high pressure liquid13N-NH3Radiochemical purity
DOI:
10.3760/cma.j.issn.1673-4114.2015.03.004
摘要:
目的 建立新的快速鉴别正电子显像剂13N-氨水及测定其放射化学纯度的有效方法。方法 采用高效液相色谱-紫外检测器联用放射性流动计数器(HPLC-UV-Flow-Count)的方法,以12.5%的氨水和0.1 mg/ml硝酸钠(NaNO3)作为标准液,AQ-C18柱为分离柱,流动相为5 mmol/L的辛烷磺酸钠水溶液和乙腈(体积比为3:1),流速为0.5 ml/min,紫外吸收波长为210 nm。将氨水和硝酸钠标准液进样检测,得到它们的色谱图后对比保留时间进行鉴别。结果 在上述的洗脱条件下,氨水和硝酸钠的UV保留时间分别为2.3和1.4 min,13N-氨水和杂质13N-NO-x的放射性保留时间分别为5.8和3.1 min,两种物质分离良好。放射性显像剂13N-氨水的放射化学纯度大于95%,符合临床应用的要求。结论 该方法简单、快速,适合半衰期极短的13N-氨水正电子显像剂的鉴别和放射化学纯度的测定。
Abstract:
ObjectiveTo establish a new, rapid, and effective method to identify 13N-NH3 and analyze its radiochemical purity.MethodsThe chromatographic method of high performance liquid chromatography-ultraviolet(HPLC-UV)-flow-count was performed using a C18 column with mobile phase of acetonitrile and 5 mmol/L sodium 1-octanesulfonate solution(V/V=1/3)at a flow rate of 0.5 ml/min and a wavelength of 210 nm. 15% 13N-NH3 and 0.1 mg/ml NaNO3 were detected separately, and their retention times were compared and identified.ResultsThe UVretentiontimesof NH3 and NaNO3 were 2.3 and 1.4 min, respectively, and the retention times of 13N-NH3 and 13N-NOx- were 5.8 and 3.1 min, respectively. The established separation method using HPLC-UV-flow-count showed good efficiency.ConclusionHPLC-UV-flow-count is a simple, valid, and rapid method to identify 13N-NH3 and analyze its radiochemical purity.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2014-09-04。
基金项目:广东省企业技术研发与升级改造专项资金(项目编号:2013B021800191)
通讯作者:何山震,Email:candido.he@163.com
更新日期/Last Update: 1900-01-01