[1]范美婷,马云,何淑雅.耐辐射奇球菌中多效蛋白PprA功能的研究进展[J].国际放射医学核医学杂志,2015,39(2):149-153.[doi:10.3760/cma.j.issn.1673-4114.2015.02.010]
　Fan Meiting,Ma Yun,He Shuya.Research progress and prediction on pleiotropic protein PprA from Deinococcus radiodurans[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(2):149-153.[doi:10.3760/cma.j.issn.1673-4114.2015.02.010]
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
39
期数:
2015年第2期
页码:
149-153
栏目:
出版日期:
2015-03-25

文章信息/Info

Title:
Research progress and prediction on pleiotropic protein PprA from Deinococcus radiodurans
作者:
范美婷1;  马云2; 3;  何淑雅1; 2; 3
1. 南华大学公共卫生学院放射医学教研室, 衡阳, 421001;
2. 南华大学生物化学与分子生物学教研室, 衡阳, 421001;
3. 湖南省分子靶标新药协同创新中心, 衡阳, 421001
Author(s):
Fan Meiting1;  Ma Yun2; 3;  He Shuya1; 2; 3
Department of Radiation Medicine, School of Public Health, University of South China, Hengyaug, 421001, China
关键词:
PprA蛋白; DNA损伤修复; 基因组稳定; 辐射; 电离; 生物信息学; 耐辐射奇球菌
Keywords:
PprA protein; DNA damage repair; Genome stability; Radiation;  onizing; Bioinformatics; Deinococcus radiodurans
DOI:
10.3760/cma.j.issn.1673-4114.2015.02.010
摘要:
耐辐射奇球菌(DR)是研究辐射抗性的模式生物,PprA蛋白(pleiotropic protein promoting DNA repair)是DR中一种特有的、促进DNA修复的多效蛋白。笔者对PprA蛋白在DNA损伤修复和维持基因组稳定性等方面的功能进行了综述,另通过运用生物信息学方法预测其结构域及与PprA蛋白相互作用的蛋白,进一步了解其发挥作用的机制和途径。
Abstract:
Deinococcus radiodurans (DR) is a model organism to study radiation resistance.PprA protein (pleiotropic protein promoting DNA repair) is a unique,pleiotropic protein promoting DNA repair in DR.This article reviewed its possible function in DNA damage repairing,maintenance of genome stability and other aspects.Furthermore,we analyze its domain of pprA genes and by bioinformatics and predict interaction protein with PprA protein,in order to predict its function and understand the mechanisms and pathways it plays.

参考文献/References:

[1] Anderson AW,Nordan HC,Can RF,et al.Studies on a radio-resis-tant micrococcus Isolation,morphology,cultural characteristics,and resistance to gamma radiation[J].Food Techmol,1956,10(3):575-578.
[2] Minton KW.Repair of ionizing radiation damage in the radiation resistant bacterium Deinococccus radiodurans[J].Mutat Res,1996,363(1):1-7.
[3] Repar J,Cvjetan S,Slade D,et al.RecA protein assures fidelity of DNA repair and genome stability in Deinococcus radiodurans[J].DNA Repair (Amst),2010,9(11):1151-1161.
[4] Levin-Zaidman S,Englander J,Shimoni E,et al.Ringlike structure of the Deinococcus radiodurans genome:a key to radioresistance?[J].Science,2003,299(564):254-256.
[5] Ghosal D,Omelchenko MV,Gaidamakova EK,et al.How radiation kills cells:survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress[J].FEMS Microbiol Rev,2005,29(2):361-375.
[6] Slade D,Lindner AB,Paul G,et al.Recombination and replication in DNA repair of heavily irradiated Deinococcus radiodurans[J].Cell,2009,136(6):1044-1055.
[7] White O,Eisen JA,Heidelberg JF,et al.Genome se2 quence of the radioresistant bacterium Deinococcus radiodurans R1[J].Science,1999,286(5444):1571-1577.
[8] 杜泽吉,田海林,鸣海一成,等.抗辐射菌Deinococcus radiodu-rans一种新的DNA修复基因pprA的分子克隆测序及特性研究(英文)[J].辐射研究与辐射工艺学报,1998,16(4):26-32.
[9] Devigne A,Mersaoui S,Bouthier-De-La-Tour C,et al.The PprA protein is required for accurate cell division of γ-irradiated Deinococcus radiodurans bacteria[J].DNA Repair (Amst),2013,12(4):265-272.
[10] Narumi I,Satoh K,Cui S,et al.PprA:a novel protein from Deinococcus radiodurans that stimulates DNA ligation[J].Mol Mi-crobiol,2004,54(1):278-285.
[11] Zahradka K,Slade D,Bailone A,et al.Reassembly of shattered chromosomes in Deinococcus radiodurans[J].Nature,2006,443(7111):569-573.
[12] Tanaka M,Earl AM,Howell HA,et al.Analysis of deinococcus ra-diodurans’s transcriptional response to ionizing radiation and desic-cation reveals novel proteins that contribute to extreme radioresis-tance[J].Genetics,2004,168(1):21-33.
[13] Liu Y,Zhou J,Omelchenko MV,et al.Transcriptome dynamics of Deinococcus radiodurans recovering from ionizing radiation[J].Proc Natl Acad Sci USA,2003,100(7):4191-4196.
[14] Kota S,Charaka VK,Misra HS.PprA,a pleiotropic protein for ra-dioresistance,works through DNA gyrase and shows cellular dy-namics during postirradiation recovery in Deinococcus radiodurans[J].J Genet,2014,93(2):349-354.
[15] Lu H,Gao G,Xu G,et al.Deinococcus radiodurans PprI switches on DNA damage response and cellular survival networks after radi-ation damage[J].Mol Cell Proteomics,2009,8(3):481-494.
[16] Lu H,Chen H,Xu G,et al.DNA binding is essential for PprI func-tion in response to radiation damage in Deinococcus radiodurans[J].DNA Repair (Amst),2012,11(2):139-145.
[17] Das AD,Misra HS.Characterization of DRA0282 from Deinococcus radiodurans for its role in bacterial resistance to DNA damage[J].Microbiology,2011,157(Pt 8):2196-2205.
[18] Satoh K,Ohba H,Sghaier H,et al.Down-regulation of radioresis-tance by LexA2 in Deinococcus radiodurans[J].Microbiology,2006,152(Pt 11):3217-3226.
[19] Ohba H,Satoh K,Sghaier H,et al.Identification of PprM:a mod-ulator of the PprI-dependent DNA damage response in Deinococcus radiodurans[J].Extremophiles,2009,13(3):471-479.
[20] Sheng D,Jao J,Li M,et al.RecX is involved in the Switch between DNA damage response and normal metabolism in D.radiodurans[J].J Biochem,2009,146(3):337-342.
[21] Kota S,Misra HS.PprA:A protein implicated in radioresistance of Deinococcus radiodurans stimulates catalase activity in Escherichia coli[J].Appl Microbiol Biotechnol,2006,72(4):790-796.
[22] Rajpurohit YS,Misra HS.Structure-function study of deinococcal serine/threonine protein kinase implicates its kinase activity and DNA repair protein phosphorylation roles in radioresistance of Deinococcus radiodurans[J].Int J Biochem Cell Biol,2013,45(11):2541-2552.
[23] Adachi M,Hirayama H,Shimizu R,et al.Interaction of double-stranded DNA with polymerized PprA protein from Deinococcus ra-diodurans[J].Protein Sci,2014,23(10):1349-1358.
[24] Kota S,Charaka VK,Ringgaard S,et al.PprA contributes to Deinococcus radiodurans resistance to nalidixic acid,genome maintenance after DNA damage and interacts with deinococcal topoisomerases[J/OL].PLoS One,2014,9(1):e85288[2015-02-01].http://journals.plos.org/plosone/article?id=10.1371/journal.
[25] Harris DR,Ngo KV,Cox MM.The stable,functional core of DdrA from Deinococcus radiodurans R1 does not restore radioresistance in vivo[J].J Bacteriol,2008,190(19):6475-6482.
[26] Harris DR,Tanaka M,Saveliev SV,et al.Preserving genome in-tegrity:the DdrA protein of Deinococcus radiodurans R1[J/OL].PLoS Biol,2004,2(10):e304[2015-02-01].http://journals.plos.org/polsiology/article?id=10.1371/journal.pbio.0020304.
[27] Selvam K,Duncan JR,Tanaka M,et al.DdrA,DdrD,and PprA:components of UV and mitomycin C resistance in Deinococcus ra-diodurans R1[J/OL].PLoS One,2013,8(7):e69007[2015-02-01].http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0069007.
[28] Devigne A,Ithurbide S,Bouthier de la Tour C,et al.DdrO is an es-sential protein that regulates the radiation desiccation response and the apoptotic-like cell death in the radioresistant Deinococcus ra-diodurans bacterium[J/OL].Mol Microbiol,2015[2015-03-05].http://www.ncbi.nlm.nih.gov/pubmed/25754115.[Epub ahead of print Mar J,2015].
[29] Selvam K,Duncan JR,Tanaka M,et al.DdrA,DdrD,and PprA:components of UV and mitomycin C resistance in Deinococcus ra-diodurans R1[J/OL].PLoS One,2013,8(7):e69007[2015-02-01].http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0069007.

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备注/Memo

备注/Memo:
收稿日期:2015-03-07。
基金项目:国家自然科学基金(81272993)
通讯作者:何淑雅,Email:skyhe2000@Hotmail.com
更新日期/Last Update: 1900-01-01