[1]张俊伶,路璐,李德冠,等.基因芯片表达分析4Gyγ射线对小鼠骨髓c-kit阳性细胞影响[J].国际放射医学核医学杂志,2015,39(2):116-120.[doi:10.3760/cma.j.issn.1673-4114.2015.02.003]
　Zhang Junling,Lu Lu,Li Deguan,et al.Expression changes of genes in c-kit positive cells after 4Gy γ-ray irradiation: agene chip analysis[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(2):116-120.[doi:10.3760/cma.j.issn.1673-4114.2015.02.003]
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
39
期数:
2015年第2期
页码:
116-120
栏目:
出版日期:
2015-03-25

文章信息/Info

Title:
Expression changes of genes in c-kit positive cells after 4Gy γ-ray irradiation: agene chip analysis
作者:
张俊伶;  路璐;  李德冠;  孟爱民
中国医学科学院放射医学研究所, 天津市放射医学与分子核医学重点实验室, 天津, 300192
Author(s):
Zhang Junling;  Lu Lu;  Li Deguan;  Meng Aimin
Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin 300192, China
关键词:
原癌基因蛋白质c-kit; 寡核苷酸序列分析; 辐射; 电离; 细胞代谢
Keywords:
Proto-oncogene proteins c-kit; Oligonucleotide array sequence analysis; Radiation;  ionizing; Metabolic process
DOI:
10.3760/cma.j.issn.1673-4114.2015.02.003
摘要:
目的 利用基因芯片技术分析经4Gyγ射线照射后c-kit(CD117,正常表达于干祖细胞表面的细胞因子受体)阳性细胞与代谢过程相关的基因及信号通路表达变化。方法 利用磁珠分选系统分选出骨髓c-kit阳性细胞。实验分为对照组、4Gy照射组。取1×106个c-kit阳性细胞进行照射,照射剂量率为0.99 Gy/min.照射后将细胞培养18h后取出,进行全基因组的高通量基因芯片检测,并进行Gene Ontology聚类分析和Kyoto Encyclopedia of Genes and Genomes信号通路分析。结果 4Gyγ射线照射引起c-kit阳性细胞包括Pld5、Neu2、Bpgm、Alas2、Satl1、Rdh16、Mccc1、Sat2、Smug1、Cml5、Adhfe1、Idh3a、Slc27a5在内的13个基因表达上调3倍以上,包括Acss2、Phgdh、Psat1、Glb1、Gpam、Dus3l、Fuca2、Impdh1、Dlat、Glb1、Enpp4、Prim2、Mettl10、Slc27a2、Dera、Qdpr、Dus1l、Cdyl2、Dhodh、Srr、Spr、Mical2在内的22个基因表达下调3倍以上,这些基因主要参与嘌呤代谢、嘧啶代谢、三羧酸循环等信号通路。结论 4Gyγ射线照射能引起c-kit阳性细胞中与代谢过程相关的许多基因表达发生变化,这些变化的基因有待进一步的实验验证。
Abstract:
Objective To analyze the metabolic process-related genes and pathway variations in the c-kit(CD117, a cytokine receptor expressed on the surface of hematopoietic stem cells) positive cells after 4Gy irradiation by using gene chip technology.Methods c-kit positive cells were sorted by microbead separation system.Two groups were involved:control group and 4Gy irradiation group.A total of 1×106 c-kit positive cells received 4Gy irradiation at a dose rate of 0.99 Gy/min.After the two groups were cultured for 18 h, all the cells were collected and detected by gene chip arrays.Data were processed and analyzed via Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways.Results After 4Gy γ-ray irradiation.13 metabolic process-related genes were up-regulated more than 3 times:Pld5, Neu2, Bpgm, Alas2, Satll, Rdhl6, Mcccl, Sat2, Smugl, Cml5, Adhfel, ldh3a, and Sle27a5.Additionally, 22 genes were down.regulated more than 3 times:Acss2, Phgdh, Psatl, Glbl, Gpam, Dus31, Fuca2, Impdhl, Dlat, Glbl, Enpp4, Prim2, MettllO, Slc27a2, Dera, Qdpr, Dusll, Cdyl2, Dhodh, Srr, Spr, and Mical2.These genes were involved in pyrimidine metabolism, purine metabolism, and citrate cycle(TCA cycle) pathways.Conclusions Many variations of genes and pathways in c-kit positive cells existed after 4Gy irradiation.However, further laboratory technology is required to confirm these variations.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-01-21。
基金项目:国家自然科学基金海外合作基金项目(项目编号:81129020);国家重点基础研究发展计划("973"项目)重大专项(项目编号:2011CB964800-G);国家自然科学基金面上项目(项目编号:81372928);国家自然科学基金青年项目(项目编号:81402633);协和青年基金资助和中央高校基本科研业务费专项资金资助项目(项目编号:33320140033)
通讯作者:孟爱民,Email:ai_min_meng@126.com
更新日期/Last Update: 1900-01-01