[1]宋梦姣,韩栋梁,葛宁,等.放疗增敏研究中人卵巢癌SKOV3乏氧细胞模型的建立方法[J].国际放射医学核医学杂志,2014,38(6):356-359.[doi:10.3760/cma.j.issn.1673-4114.2014.06.002]
　Song Meng-jiao,Han Dong-liang,Ge Ning,et al.The method of establishing the hypoxic SKOV3 cellular model in the research of radiotherapy sensitizer[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(6):356-359.[doi:10.3760/cma.j.issn.1673-4114.2014.06.002]
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
38
期数:
2014年第6期
页码:
356-359
栏目:
出版日期:
2014-11-25

文章信息/Info

Title:
The method of establishing the hypoxic SKOV3 cellular model in the research of radiotherapy sensitizer
作者:
宋梦姣1; 3;  韩栋梁2;  葛宁2;  张继青2;  曹金发1;  刘宏1
1. 广州军区武汉总医院药剂科, 430070;
2. 广州军区武汉总医院放疗中心, 430070;
3. 珠海市人民医院药学部, 519000
Author(s):
Song Meng-jiao1; 3;  Han Dong-liang2;  Ge Ning2;  Zhang Ji-qing2;  Cao Jin-fa1;  Liu Hong1
Department of Pharmacy, Wuhan General Hospital of Guangzhou Military, Wuhan 430070, China
关键词:
卵巢肿瘤; SKOV3细胞株; 乏氧细胞; 放疗增敏
Keywords:
Breast neoplasms; SKOV3 cell line; Hypox cell; Radiotherapy sensitizer
DOI:
10.3760/cma.j.issn.1673-4114.2014.06.002
摘要:
目的 探讨适用于放疗增敏研究中人卵巢癌SKOV3乏氧细胞模型的建立方法。方法 将人卵巢癌细胞株SKOV3分为常氧对照组、二氯化钴组和环境乏氧组,各组均在X射线单次照射(0、2、4、6、8 Gy)后72 h,应用噻唑蓝法检测细胞增殖。结果 与常氧对照组未照射细胞相比,二氯化钴组和环境乏氧组未照射细胞的细胞增殖率均显着降低(t=24.789、196.960,P均<0.01);与同组未照射细胞相比,常氧对照组与二氯化钴组接受不同剂量的X射线单次照射后,其细胞存活率均显着性下降(F=2263.039、3672.044,P均<0.01),且放射剂量越大,其细胞存活率越低,而环境乏氧组无显着性变化(F=1.412,P>0.05);与常氧对照组、二氯化钴组的同剂量照射细胞相比,环境乏氧组细胞存活率均显着升高(2Gy:F=61.125;4Gy:F=181.825;6Gy:F=373.830;8Gy:F=2425.510,P均<0.01).结论 环境乏氧组的细胞对射线产生了强烈的抵抗性,即放射敏感性显着性降低,射线对其杀伤力减弱,且所获得的乏氧细胞模型明显优于二氯化钴组,因此环境乏氧比二氯化钴更适合作为放疗增敏研究中人卵巢癌SKOV3乏氧细胞模型的建立方法。
Abstract:
Objective To investigate the suitable method of establishing the hypoxic SKOV3 cellular model in the research of radiotherapy sensitizer.Methods Human ovarian cancer cell line SKOV3 were divided into three groups:normoxic control group,cobalt chloride group and environmental hypoxia group.Cell proliferation of each group 72 h after single X-ray irradiation (0,2,4,6,8 Gy)were detected by MTT assay.Results Cell proliferation of non-irradiated cells in cobalt chloride group and environmental hypoxia group were significantly lower than that in normoxic control group (t=24.789,196.960,both P<0.01).Cell viability of the cells in normoxic control group and cobalt chloride group were significantly decreased after receiving a single X-ray irradiation of different doses compared with non-irradiated cells in the same group (F=2263.039,3672.044,both P<0.01)and their cell viability were decreased with the increase of radiation dose,while environmental hypoxia group had no significant change (F=1.412,P>0.05).The cell viability of irradiated cells in environmental hypoxia group were significantly higher than the irradiated cells with the same radiation in normoxic control group and cobalt chloride group (2 Gy:F=61.125; 4 Gy:F=181.825; 6 Gy:F=373.830; 8 Gy:F=2425.510,all P<0.01).Conclusions Cells in environmental hypoxia group were very resistant to radiation.Their radiation sensitivity strikingly decreased and the killing effect of radiation on them was weak.The hypoxic cellular model of environmental hypoxia group was obviously superior to cobalt chloride group.Environmental hypoxia method was more suitable to establish the hypoxic SKOV3 cellular model in the research of radiotherapy sensitizer compared with cobalt chloride.

参考文献/References:

[1] Harada H.How can we overcome tumor hypoxia in radiation therapy?[J].J Radiat Res,2011,52(5):545-556.
[2] Meena RC,Kumar N,Nath S,et al.Homologous recombination is activated at early time points following exposure to cobalt chloride induced hypoxic conditions in Saccharomyces cerevisiae[J].Indian J Microbiol,2012,52(2):209-214.
[3] Javvadi P,Segan AT,Tuttle SW,et al.The chemopreventive agent curcumin is a potent radiosensitizer of human cervical tumor cells via increased reactive oxygen species production and overactivation of the mitogen-activated protein kinase pathway[J],Mol Pharmacol,2008,73(5):1491-1501.
[4] Qiu L,Ding X,Zhang Z,et al.Copper is required for cobalt-induced transcriptional activity of hypoxia-inducible factor-1[J].J Pharmacol Exp Ther,2012,342(2):561-567.
[5] Singh-Gupta V,Zhang H,Banerjee S,et al.Radiation-induced HIF-1α cell survival pathway is inhibited by soy isoflavones in prostate cancer cells[J].Int J Cancer,2009,124(7):1675-1684.

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备注/Memo

备注/Memo:
收稿日期:2014-03-12。
基金项目:湖北省自然科学基金(2014CFCl053)
通讯作者:刘宏(Email:honguil@163.com)
更新日期/Last Update: 1900-01-01