[1]张晓东,陈婕,吴迪,等.金纳米颗粒修饰及其对肝癌细胞生长和辐射损伤的影响[J].国际放射医学核医学杂志,2014,38(3):157-160,189.[doi:10.3760/cma.j.issn.1673-4114.2014.03.004]
 Zhang Xiaodong,Chen Jie,Wu Di,et al.Effect of Au nanoparticles modified with sulfhydryl-polyethylene glycol on liver cancer cell proliferation and radiation damage[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(3):157-160,189.[doi:10.3760/cma.j.issn.1673-4114.2014.03.004]
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金纳米颗粒修饰及其对肝癌细胞生长和辐射损伤的影响(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
38
期数:
2014年第3期
页码:
157-160,189
栏目:
出版日期:
2014-05-25

文章信息/Info

Title:
Effect of Au nanoparticles modified with sulfhydryl-polyethylene glycol on liver cancer cell proliferation and radiation damage
作者:
张晓东 陈婕 吴迪 沈秀 宋莎莎 龙伟 王浩 刘培勋 樊赛军 孙元明
300192 天津, 北京协和医学院中国医学科学院放射医学研究所, 天津市放射医学与分子核医学重点实验室
Author(s):
Zhang Xiaodong Chen Jie Wu Di Shen Xiu Song Shasha Long Wei Wang Hao Liu Peixun Fan Saijun Sun Yuanming
Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China
关键词:
聚乙烯二醇类金纳米颗粒放射增敏肝癌细胞
Keywords:
Polyethylene glycolsAu nanoparticlesRadiosensitiveLiver cancer cell
DOI:
10.3760/cma.j.issn.1673-4114.2014.03.004
摘要:
目的 用巯基-聚乙二醇(SH-PEG)对金纳米颗粒(AuNPs)进行化学修饰(PEG-AuNPs)并分析其对肝癌细胞存活率和辐射对肝癌细胞作用的影响。方法 制备的AuNPs用SH-PEG进行化学修饰,用透射电子显微镜观测PEG-AuNPs的大小及肝癌细胞对其的摄取,应用Cell Titer-Glo发光法和克隆形成实验分别分析PEG-AuNPs对肝癌细胞的生长抑制作用和辐射对肝癌细胞作用的影响。结果 制备的PEG-AuNPs的尺寸分别为14.4 nm和30.5 nm。30.5 nm PEG-AuNPs更容易被肝癌细胞摄取,表现出明显地抑制肝癌细胞生长的作用和提高辐射对肝癌细胞的杀伤作用。结论 AuNPs经过SH-PEG化学修饰,30.5 nm PEG-AuNPs对肝癌细胞的生长抑制作用和提高辐射杀伤肝癌细胞的作用强于14.4 nm PEG-AuNPs。
Abstract:
Objective Au nanoparticles (AuNPs) was chemically modified with sulfhydryl-polyethylene glycol (SH-PEG) and its effects on liver cancer cell survival and radiation damage were assessed.Methods PEG-AuNPs were chemically fabricated with SH-PEG and AuNPs.The size of PEGAuNPs and uptake by liver cancer cell were observed by field-emission transmission electron microscopy (TEM).The Cell Titer-Glo luminescent cell viability assay and cell colonies were used to examine effect of PEG-AuNPs on liver cancer cells’survival and radiation damage.Results 14.4 nm and 30.5 nm PEGAuNPs were obtained by above fabrication.30.5 nm PEG-AuNPs were presented more absorption,inhibi-tion the survival and enhance radiation effect to liver cancer cell.Conclusions After modification with SH-PEG,larger PEG-AuNPs with 30.5 nm showed stronger inhibition in liver cancer cellular survival and promoted liver cancer cells sensitive to radiation.

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相似文献/References:

[1]陈婕,张晓东,吴迪,等.巯基-聚乙二醇修饰的不同尺寸金纳米颗粒的制备和光学特性[J].国际放射医学核医学杂志,2013,37(1):16.[doi:10.3760/cma.j.issn.1673-4114.2013.01.005]
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[2]张晓东,宋莎莎,陈婕,等.15nm聚乙二醇保护的Au纳米颗粒对HepG2细胞的放射增敏作用[J].国际放射医学核医学杂志,2014,38(1):5.[doi:10.3760/cma.j.issn 1673-4114.2014.01.002]
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备注/Memo

备注/Memo:
收稿日期:2013-12-25。
基金项目:国家自然科学基金(81000668);天津市自然科学基金(13JCQNJC13500);中国医学科学院放射医学研究所所基金(SF1207和SZ1336);北京协和医学院协和新星基金(人1256);北京协和医学院青年基金(院1343)
通讯作者:张晓东,Email:xiaodongzhang@irm-cams.ac.cn;孙元明,Email:yuanmings1962@163.com
更新日期/Last Update: 1900-01-01