[1]王芹,刘晓秋,徐畅,等.抗辐射小鼠cDNA文库的全长cDNA功能鉴定[J].国际放射医学核医学杂志,2013,37(5):262-264.[doi:10.3760/cma.j.issn.1673-4114.2013.05.002]
　WANG Qin,LIU Xiao-qiu,XU Chang,et al.The function analysis of full-length cDNA sequence from IRM-2 mouse cDNA library[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(5):262-264.[doi:10.3760/cma.j.issn.1673-4114.2013.05.002]
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
37
期数:
2013年第5期
页码:
262-264
栏目:
论著
出版日期:
2013-09-25

文章信息/Info

Title:
The function analysis of full-length cDNA sequence from IRM-2 mouse cDNA library
作者:
王芹;  刘晓秋;  徐畅;  杜利清;  孙志娟;  王彦;  刘强;  宋力;  李进;  樊飞跃
300192 天津, 北京协和医学院中国医学科学院放射医学研究所, 天津市分子核医学重点实验室
Author(s):
WANG Qin;  LIU Xiao-qiu;  XU Chang;  DU Li-qing;  SUN Zhi-juan;  WANG Yan;  LIU Qiang;  SONG Li;  LI Jin;  FAN Fei-yue
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China
关键词:
寡核苷酸序列分析; 基因文库; IRM-2小鼠
Keywords:
Oligonucleotide array sequence analysis; Gene library; IRM-2 mice
DOI:
10.3760/cma.j.issn.1673-4114.2013.05.002
摘要:
目的 鉴定IRM-2小鼠cDNA文库的全长cDNA功能。方法 以IRM-2小鼠的21条表达序列标签为引物进行PCR,从IRM-2小鼠全长cDNA文库中测定cDNA克隆的序列.观察小鼠胚胎成纤维细胞经6.5 Gy γ射线照射后全长cDNA的表达和全长cDNA转染对辐射敏感细胞共济失调性毛细血管扩张症(AT)5B1VA细胞生长的影响。结果 小鼠胚胎成纤维细胞经照射后,IRM-2小鼠4号、5号和2号cDNA表达水平均高于其亲本ICR小鼠和615小鼠。将全长cDNA转染AT5B1VA细胞,经照射后4号、5号和2号cDNA细胞存活率较高。结论 IRM-2小鼠4号、5号和2号全长cDNA具有较强的抗辐射功能。
Abstract:
Objective To identify the function of full-length cDNA sequence from IRM-2 mouse cDNA library.Methods Full-length cDNA products were amplified by PCR from IRM-2 mouse cDNA library according to twenty-one pieces of expressed sequence tag.The expression of full-length cDNAs were detected after mouse embryonic fibroblasts were exposed to 6.5 Gy γ-ray radiation.And the effect on the growth of radiosensitivity cells AT5B1VA transfected with full-length cDNAs was investigated.Results The expression of No.4, 5 and 2 full-length cDNAs from IRM-2 mouse were higher than that of parental ICR and 615 mouse after mouse embryonic fibroblasts irradiated with γ-ray radiation.And the survival rate of AT5B1VA cells transfected with No.4, 5 and 2 full-length cDNAs was high.Conclusion No.4, 5 and 2 full-length cDNAs of IRM-2 mouse are of high radioresistance.

参考文献/References:

[1] 王芹,岳井银,李进,等.电离辐射诱发小鼠脾细胞DNA链断裂及修复.中国辐射卫生,2007,16(1):17-20.
[2] 王芹,岳井银,李进,等.辐射抗性小鼠与其亲代差异基因的表达序列标签.苏州大学学报:医学版,2009(1):1-3.
[3] 王芹,李进,宋力,等.IRM-2小鼠全长cDNA文库的构建及鉴定.中华放射医学与防护杂志,2010:30(3):39-42.
[4] 王芹,李进,张恒,等.IRM-2小鼠胚胎成纤维细胞的原代培养和生物学特性.苏州大学学报:医学版,2010(1):1-3.
[5] 王芹,刘晓秋,李进,等.IRM-2小鼠全长cDNA编码蛋白质分析.国际放射医学核医学杂志,2012,36(3):157-159.
[6] Xiong YH, Xu Y, Lai WH, et al. Cloning and sequence analysis of the full-length cDNA of a novel yp05 gene associated with citrinin production in Monascus aurantiacus. Biomed Environ Sci, 2007,20(2):135-140.
[7] Qi JL, Zhang WJ, Liu SH, et al. Expression analysis of light-regulated genes isolated from a full-length-enriched cDNA library of Onosma paniculatum cell cultures. J Plant Physiol, 2008, 165(14):1474-1482.
[8] Gao PF, Cao GQ, Zhao HT, et al. Molecular cloning and characteriza-tion of pigeon(Columba liva)ubiquitin and ubiquitin-conjugating enzyme genes from pituitary gland library. Int J Biol Sci, 2009,5(1):34-43.
[9] 饶亚岚,陈肖华,王琳,.7Gyγ射线照射后4h小鼠骨髓差异表达基因的初步研究.国际放射医学核医学杂志,2007,31(4):245-247.
[10] 李智恒,李雨.C57小鼠受照后胸腺细胞基因表达谱的变化.国际放射医学核医学杂志,2007,31(4):240-244.
[11] Thacker J, Zdzienicka MZ. The mammalian XRCC genes:their roles in DNA repair and genetic stability. DNA Repair (Amst),2003,2(6):655-672.
[12] 史芳,王芳.ATM基因与AT细胞辐射敏感性的研究进展.国际放射医学核医学杂志,2007,31(4):248-250.
[13] 郭阳.DNA链间交联的修复与辐射敏感性.国际放射医学核医学杂志,2007,31(6):371-372.

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[1]张俊伶,路璐,李德冠,等.基因芯片表达分析4Gyγ射线对小鼠骨髓c-kit阳性细胞影响[J].国际放射医学核医学杂志,2015,39(2):116.[doi:10.3760/cma.j.issn.1673-4114.2015.02.003]
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备注/Memo

备注/Memo:
收稿日期:2012-09-07。
基金项目:国家自然科学基金(30870583);中国医学科学院放射医学研究所学科发展基金(SF0822)
通讯作者:刘强,Email:liuqiang@irm-cams.ac.cn
更新日期/Last Update: 1900-01-01