[1]李超,李莉,许昌韶,等.脑胶质瘤SHG-44细胞株照射后子代辐射敏感性[J].国际放射医学核医学杂志,2008,32(4):236-239.
　LI Chao,LI Li,XU Chang-shao,et al.Radiosensitivity of filial generation from irradiated human glioma cell line SHG-44[J].International Journal of Radiation Medicine and Nuclear Medicine,2008,32(4):236-239.
点击复制

脑胶质瘤SHG-44细胞株照射后子代辐射敏感性(/HTML)
分享到：

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
32
期数:
2008年第4期
页码:
236-239
栏目:
放射生物学
出版日期:
1900-01-01

文章信息/Info

Title:
Radiosensitivity of filial generation from irradiated human glioma cell line SHG-44
作者:
李超1;  李莉2;  许昌韶1;  周菊英2;  徐晓婷2;  俞志英2
1. 215021 上海, 上海交通大学医学院苏州九龙医院放疗科;
2. 215006 苏州, 苏州大学附属第一医院放疗科
Author(s):
LI Chao1;  LI Li2;  XU Chang-shao1;  ZHOU Ju-ying2;  XU xiao-ting2;  YU Zhi-ying2
1. Department of Radiation, Suzhou Kowloon Hospital, Shanghai Jiaotong University Medical School Suzhou 215021, China;
2. Department of Radiation, The First Hospital Affiliated to Soochow University, Suzhou 215006, China
关键词:
神经胶质瘤; 肿瘤细胞; 培养的; 辐射耐受性; 细胞周期
Keywords:
Glioma; Tumor cells; cultured; Radiation tolerance; Ceil cycle
摘要:
目的 探讨脑胶质瘤SHG-44细胞株照射子代生长特性及辐射敏感性变化。方法 培养人脑胶质瘤SHG-44细胞株照射后的存活子代细胞,测定其群体倍增时间,并进行集落形成实验和流式细胞仪检测,分析其辐射敏感性和细胞周期变化。结果 SHG-44细胞群体倍增时间为(22.78±2.61) h,SHG-44细胞经6 MV X射线10 Gy照射后存活子代SHG-44.10细胞群体倍增时间为(30.46±2.73) h (F=7.878,P<0.05)。SHG-44细胞和SHG-44.10细胞再次2 Gy照射后的存活分数分别为70.8%、80.6%。与SHG-44细胞相比,SHG-44-10细胞在G2/M期比例减少,S期比例增多。结论 SHG-44细胞照射后存活子代细胞增殖延缓,辐射敏感性下降。
Abstract:
Objective To investigate the radiosensitivity and growth features of the progeny of irradiated human glioma cell line SHG-44.Methods The SHG-44 cells were irradiated with 6 MV X ray and the progeny of the cells were cultured.The population doubling time (PDT) was detected pre-and postirradiation. The radiosensitivity of the progeny of irradiated human glioma cell line SHG-44 was measured by clone forming assay.The cell cycle distribution was analysed by flow eytometry.Results The PDT of SHG-44 cells before irradiation Was (22.78±2.61)h.The PDT of irradiated SHG-44 ceils at dose of 10 Gy were (30.46±2.73)h (F=7.878,P<0.05).The surviving fraction of SHG-44 cell was 70.8%,SHG-44-10 cell was 80.6%.SHG-44 cells showed significant G2/M phase decrease and S phase arrest after irradiation. Conclusion Growth delay and declined radiosensitivity are confirmed in the progeny of irradiated SHG-44 cells.

参考文献/References:

[1] Li X, Pontén A, Aase K, et al. PDGF-C is a new protease-activated ligand for the PDGF a-receptor[J]. Nat Cell Biol, 2000, 2(5): 302309.
[2] Bergsten E, Uutela M, Li X, et al. PDGF-D is a specific, protease-activated ligand for the PDGF β-receptor[J]. Nat Cell Biol, 2001, 3(5): 512-516.
[3] Fredriksson L, Li H, Eriksson U. The PDGF family: four gene products form five dimeric isof orraa[J]. Cytokine Growth Factor Rev, 2004, 15(4): 197-204.
[4] Uutela M, Laurén J, Bergsten E, et al. Chromosomal location, exon structure, and vascular expression patterns of the huriian PDGFC and PDGFC genes[J], Circulation, 2001,103(18): 2242-2247.
[5] Hcldin CH, Westermark B. Mechanism of action and in vivo role of platelet-derived growth factor[J], Physiol Rev, 1999, 79(4): 1283-1316.
[6] LI X, Eriksson U. Novel PDGF family members: PDGF-C and PDGF-D[J]. Cytokine Growth Factor Rev, 2003,14(2): 91-98.
[7] Heldin CH, Eriksson U, Ostman A. New members of the platelet-derived growth factor family of mitogens[J]. Arch Biochem Biophys, 2002. 398(2): 284-290.
[8] Cools J, DeAngelo DJ, Cotlib J, et al. A tyrosine kinase created by fusion of the PDGFRA and FIP1L1 genes as a therapeutic target of imatinib in idiopathic hypereosinophilic syndrome[J]. N Engl J Med, 2003,348(13): 1201-1214.
[9] MacDonald TJ, Brown KM, LaFleur B, et al. Expression profiling of medulloblastoma: PDGFRA and the RAS/MAPK pathway as therapeutic targets for metastatic disease[J], Nat Genet, 2001, 29(2): 143-152.
[10] Ostman A. PDGF receptors-mediators of autocrine tumor growth and regulators of tumor vasculature and stroma[J]. Cytokine Growth Factor Rev, 2004, 15(4): 275-286.
[11] Pietras K, Sjöblom T, Rubin K, et al. PDGF receptors as cancer drug targets[J]. Cancer Cell, 2003,3(5): 439-443.
[12] Tallquist M, Kazlauskas A. PDGF signaling in cells and mice[J]. Cytokine Growth Factor Rev, 2004,15(4): 205-213,
[13] Jones SM, Kazlauskas A. Growth factor-dependent signaling and cell cycle progression[J]. FEBS Lett, 2001,490(3):110-116.
[14] Vantlei. M, Huntgeburth M, Caglayan E, et al. PI3-kinase/Akt-dependent antiapoptotic signaling by the PDGFa receptor is negatively regulated by Src family kinases[J]. FEBS Lett, 2006, 580(30): 6769-6776.

相似文献/References:

[1]程勇军,赵晋华.东亚钳蝎氯毒素在胶质瘤靶向显像与治疗中的研究进展[J].国际放射医学核医学杂志,2015,39(1):32.[doi:10.3760/cma.j.issn.1673-4114.2015.01.008]
　Cheng Yongjun,Zhao Jinhua.Development of research in targeting image and therapy of gliomas using Buthus martensii Karsch chlorotoxin[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(4):32.[doi:10.3760/cma.j.issn.1673-4114.2015.01.008]
[2]高艳,赵晋华.PET/MRI融合显像在脑胶质瘤诊断和治疗中的研究进展[J].国际放射医学核医学杂志,2015,39(1):71.[doi:10.3760/cma.j.issn.1673-4114.2015.01.015]
　Gao Yan,Zhao Jinhua.The advancement of PET/MRI on the diagnosis and treatment of brain gliomas[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(4):71.[doi:10.3760/cma.j.issn.1673-4114.2015.01.015]
[3]周惠君,董萍,沈国华,等.11C-蛋氨酸PET/CT显像在脑胶质瘤中的应用价值[J].国际放射医学核医学杂志,2015,39(2):161.[doi:10.3760/cma.j.issn.1673-4114.2015.02.013]
　Zhou Huijun,Dong Ping,Shen Guohua,et al.Value of 11C-methionine PET/CT imaging indiagnosing cerebral glioma[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(4):161.[doi:10.3760/cma.j.issn.1673-4114.2015.02.013]
[4]周新韩,高明勇,张仙海,等.磁敏感加权成像显示肿瘤内静脉评价胶质瘤分级[J].国际放射医学核医学杂志,2014,38(6):373.[doi:10.3760/cma.j.issn.1673-4114.2014.06.006]
　Zhou Xin-han,Gao Ming-yong,Zhang Xian-hai,et al.Values of tumor vein detected by susceptibility weighted imaging in evaluation of gliomas grade[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(4):373.[doi:10.3760/cma.j.issn.1673-4114.2014.06.006]
[5]李平,宗天舟,季晓芹,等.CD133+U87人脑胶质瘤干细胞放射敏感性和DNA双链断裂损伤修复的实验研究[J].国际放射医学核医学杂志,2013,37(3):153.[doi:10.3760/cma.j.issn.1673-4114.2013.03.007]
　LI Ping,ZONG Tian-zhou,JI Xiao-qin,et al.CD133 positive U87 glioma stem cell radiosensitivity and DNA double-strand break repair[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(4):153.[doi:10.3760/cma.j.issn.1673-4114.2013.03.007]
[6]展凤麟,汪世存,潘博,等.11C-胆碱和18F-FDG PET/CT显像在脑胶质瘤诊断中的应用[J].国际放射医学核医学杂志,2013,37(4):193.[doi:10.3760/cma.j.issn.1673-4114.2013.04.001]
　ZHAN Feng-lin,WANG Shi-cun,PAN Bo,et al.Application of 11C-choline and 18F-FDG PET/CT in diagnosing brain glioma[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(4):193.[doi:10.3760/cma.j.issn.1673-4114.2013.04.001]
[7]杨辉,张富强,李文亮,等.术中125Ⅰ粒子植入治疗复发性脑胶质瘤的临床研究[J].国际放射医学核医学杂志,2013,37(6):345.[doi:10.3760/cma.j.issn.1673-4114.2013.06.005]
　YANG Hui,ZHANG Fu-qiang,LI Wen-liang,et al.Clinical study of operation combined with 125Ⅰ seeds implantation in the treatment of recurrent cerebral glioma[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(4):345.[doi:10.3760/cma.j.issn.1673-4114.2013.06.005]
[8]乐亚丽,陈跃.多种不同PET或PET/CT分子探针在胶质瘤中的应用进展[J].国际放射医学核医学杂志,2013,37(6):364.[doi:10.3760/cma.j.issn.1673-4114.2013.06.010]
　LE Ya-li,CHEN Yue.Progress on application of different PET or PET/CT agents in glioma[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(4):364.[doi:10.3760/cma.j.issn.1673-4114.2013.06.010]
[9]刁焕荣,刘晓岚.MRI在颅内恶性胶质瘤放疗中的应用进展[J].国际放射医学核医学杂志,2011,35(3):185.[doi:10.3760/cma.j.issn.1673-4114.2011.03.013]
　DIAO Huan-rong,LIU Xiao-lan.The use of MRI in radiotherapy of brain malignant gliomas[J].International Journal of Radiation Medicine and Nuclear Medicine,2011,35(4):185.[doi:10.3760/cma.j.issn.1673-4114.2011.03.013]
[10]孙娜,赵晋华,乔文礼.胶质瘤显像剂的研究与应用进展[J].国际放射医学核医学杂志,2011,35(6):330.[doi:10.3760,cma.j.issn.1673-4114.2011.06.003]
　SUN Na,ZHAO Jin-hua,QIAO Wen-li.Advances in appUcafion of radiotracers for glioma imaging[J].International Journal of Radiation Medicine and Nuclear Medicine,2011,35(4):330.[doi:10.3760,cma.j.issn.1673-4114.2011.06.003]

备注/Memo

备注/Memo:
收稿日期:2007-11-21。
通讯作者:李莉,E-mail:lili371626077@sohu.com
更新日期/Last Update: 1900-01-01