[1]邓胜明,张玮,章斌,等.188Re-胰岛素样生长因子1类似物在荷人胰腺癌裸鼠体内分布及其显像研究[J].国际放射医学核医学杂志,2008,32(3):129-133.
　DENG Sheng-ming,ZHANG Wei,ZHANG Bin,et al.Studies on biodistribution and imaging of 188Re labeled insulin-like growth factor-1 analogue in nude mice bearing human pancreatic carcinoma[J].International Journal of Radiation Medicine and Nuclear Medicine,2008,32(3):129-133.
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188Re-胰岛素样生长因子1类似物在荷人胰腺癌裸鼠体内分布及其显像研究(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
32
期数:
2008年第3期
页码:
129-133
栏目:
实验核医学
出版日期:
1900-01-01

文章信息/Info

Title:
Studies on biodistribution and imaging of 188Re labeled insulin-like growth factor-1 analogue in nude mice bearing human pancreatic carcinoma
作者:
邓胜明;  张玮;  章斌;  罗贤文;  吴翼伟
215006 苏州, 苏州大学附属第一医院核医学科
Author(s):
DENG Sheng-ming;  ZHANG Wei;  ZHANG Bin;  LUO Xian-wen;  WU Yi-wei
Department of Nuclear Medicine, the First Affiliated Hospital of Soochow University, Suzhou 215006, China
关键词:
铼; 胰岛素样生长因子1类似物; 胰腺肿瘤; 小鼠; 裸
Keywords:
Rhenium; Insulin-like growth factor-1 receptor; Pancreatic neoplasms; Mice; nude
摘要:
目的 研究188Re标记胰岛素样生长因子l类似物(IGF-1A)在荷人胰腺癌裸鼠体内的分布及其显像。方法 ①直接法标记188Re-IGF-1A并测定标记率。②建立荷人胰腺癌Patu8988裸鼠模型。③188Re-IGF-1A经瘤内注射荷人胰腺癌裸鼠瘤内,分别于注射后15 min、1 h、4 h、24 h、3 d、5 d进行SPECT平面显像。④188ReO4-经瘤内注射后15 min、1 h、2 h、4 h、24 h进行显像,取各时间组裸鼠(n=4)脏器和肿瘤组织,计算每克组织百分注入剂量(%ID/g)及肿瘤/非肿瘤组织放射性摄取比值(T/NT)。结果 ①188Re-IGF-1A标记率为(94.07±0.32)%。②瘤内注射188Re-IGF-1A后,肿瘤部位放射性积聚量4 h内差异无统计学意义(F=1.622,P>0.05),且随时间延长,肿瘤与其他脏器的T/NT呈上升趋势,其中肿瘤/肌肉在5 d时最高,达到6531.79±4930.26.③瘤内注射188ReO4-后,在体内初始主要分布于甲状腺、胃、肿瘤、血液,随时间延长,肿瘤部位放射性计数迅速下降。④在24 h,瘤内注射188Re-IGF-1A组肿瘤及肾脏内% ID/g较188ReO4-组高,两者有统计学差异(t=5.877,t=13.287,P<0.01);两组肿瘤内% ID/g比值在24 h达到最高,为74.10倍。⑤瘤内注射188Re-IGF-1A后,SPECT平面显像见瘤内浓聚,5 d时仅见肿瘤部位显影。结论 188Re-IGF-1A对胰腺癌具有良好的亲和力,在肿瘤部位有较高的T/NT,可望作为胰腺癌治疗的药物。
Abstract:
Objective To evaluate the biodistribution and planar gamma carnera jmaging characteristics of 188Re labeled insulin-like growth factor 1 analogue(188Re-IGF-1A)in tumor-bearing mice.Methods ①To label IGF-1A with 188Re directly and to determine the labeling efficiency.②To establish nude mice model which beating human pancreatic carcinoma cell Patu8988.③To scan those nude mice at 15 min,1 h,4 h,24 h,3 d and 5 d after intratumor injection with 188Re-IGF-1A into their tumors.④To scan those nude mice at 15min,1 h,2h,4 h and24 h after intratumor injection with 188ReO4-into their tumors.To calculate the tumor to normal tissue ratio(T/NT)and the percentages of injected dose per gram tissue(%ID/g)of different organs.Results ①The labeling efficiency of 188Re-IGF-1A was(94.07±0.32)%.②The largest uptake of tumors was(42.38+17.82)%ID/g at 4 h after injection of 188Re-IGF-1A.Then the tumor to normal tissue ratios increased and the largest Rnnor to mugcle ratio was 6531.79±4930.26 at 5 d after injection.③188ReO4- wag major distributed in thyroid glands,stomachs,tumors and blood in nude mice after injection at first.Then%ID/g decreased rapidly in tumors.④The difference of%ID/g wag siguificant(t=5.877,t=13.287,P<0.01) in tumors and kidneys between 188Re-IGF-1A group than in 188ReO4- group.The largest ratio of tumors in the two groups was 74.10 at 24 h after injection.⑤After being injected,188Re-IGF-1A formed clear images in tumom.5 d later,nothing but tumors can be seen.Conclusions 188Re-IGF-1A has good affinity with human pancreatic cancer,and the tumor to muscle ratios in nude mice is high.So 188Re-IGF-1A is expected to be used for targeting therapy of human pancreatic carcinoma.

参考文献/References:

[1] Bergmann U, Funatomi H, Yokoyama M, et al. Insulin-like growth factor 1 overexpression in human pancreatic cancer:evidence for autocrine and paracrine roles[J]. Cancer Res, 1995, 55(10):2007-2011.
[2] Stoeltzing O, Liu W, Reinmuth N, et al. Regulation of hypoxiainducible facter-1, vascular endothelial growth factor, and angiogenesis by an insulin-like growth factor-Ⅰ receptor autocrine loop in human pancreatic cancer[J]. Am J Pathol, 2003, 163(3):1001-1011.
[3] Fabry M, Cabrele C, Hoeker H, et al. Differently labeled peptide ligands for rapid investigation of receptor expression on a new human glioblastoma cell line[J]. Peptides, 2000, 21(12):1885-1893.
[4] Marinelli PW, Gianoulakis C, Kar S. Effects of voluntary ethanol drinking on[125I]insulin-like growth factor-Ⅰ,[125I]insulin-like growth factor-Ⅱ and[125I]insulin receptor binding in the mouse hippocampus and cerebellum[J]. Neuroscience, 2000, 98(4):687-695.
[5] 罗朝学. 131I-抗IGF-1R单抗对肝癌的放射免疫显像实验研究[D].重庆:第三军医大学,2003.
[6] 杨晓春.联合转染反义寡核苷酸治疗胰腺癌和MRI动态观测的实验研究[D].苏州:苏州大学,2006.
[7] 章斌,吴翼伟,范我.用188Re标记IGF-1类似物的方法[J].苏州大学学报:医学版,2007,27(5):722-725.
[8] 章斌,吴翼伟,范我,等. 188Re/99Tc m-IGF-1类似物的制备及与胰腺癌细胞结合实验研究[J].中华核医学杂志,2007,27(6):338-342.
[9] Hartog H, Wesseling J, Boezen HM, et al. The insulin-like growth factor 1 receptor in cancer:Old focus, new future[J]. Eur J Cancer, 2007, 43(13):1895-1904.
[10] Karamouzis MV, Papavassiliou AG. The IGF-1 network in lung carcinoma therapeutics[J]. Trends Mol Med, 2006,12(12):595-602.

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备注/Memo

备注/Memo:
收稿日期:2007-12-09。
基金项目:苏州市社会发展项目(SSY0617);江苏省医学重点人才资助项目(35RC2002035)
通讯作者:张玮(E-mail:sdfyyzw336@hotmail.com)
更新日期/Last Update: 1900-01-01