[1]王治东,陈肖华.2DE技术及其在辐射损伤研究中的应用[J].国际放射医学核医学杂志,2003,27(1):45-47.
　WANG Zhi-dong,CHEN Xiao-hua.Progress in two-dimensional polyacrylamide gelelectrophoresis and applicationin in radiation research[J].International Journal of Radiation Medicine and Nuclear Medicine,2003,27(1):45-47.
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2DE技术及其在辐射损伤研究中的应用(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
27
期数:
2003年第1期
页码:
45-47
栏目:
放射医学
出版日期:
1900-01-01

文章信息/Info

Title:
Progress in two-dimensional polyacrylamide gelelectrophoresis and applicationin in radiation research
作者:
王治东1;  陈肖华2
1. 100850 北京, 军事医学科学院放射医学研究所放射病实验治疗研究室;
2. 100850 北京, 军事医学科学院放射医学研究所
Author(s):
WANG Zhi-dong1;  CHEN Xiao-hua2
Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China
关键词:
蛋白质分离; 二维聚丙烯酰胺; 辐射损伤
Keywords:
protein separation; two-dimensional polycrylamide gel electrophoresis; irradiation
分类号:
Q5-33;R818.23
摘要:
2DE(二维聚丙烯酰胺凝胶电泳)技术是蛋白质组学研究中的主要分离技术,其分别以蛋白质的等电点和分子质量这两个独立的参数为基础,实现蛋白质混合物的分离。2DE在探讨疾病发生机理,寻找肿瘤早期诊断指标,及药物开发研究等方面均有较好的应用,在辐射损伤研究中的应用也有广泛的前景。
Abstract:
Two-dimensional polyacrylamide gel electrophoresis is the key separation technique in proteomics research, which is designed by protein character:molecular weight and PI. Some progress has been made in disease mechanism detection, tumour indicator research and drug development. This technique also has some potential application in radiation research.

参考文献/References:

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[2] Corthals GL, Wasinger VC, Hochstrasser DF, et al. The dynamic range of protein expression:a challenge for proteomic research[J]. Electrophoresis, 2000, 21(6):1104-1115.
[3] Chevallet M, Santoni V, Poinas A, et al. New zwitterionic detergents improve the analysis of membrance proteins by two dimensional electrophoresis[J]. Electrophoresis, 1998, 19:1501.
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[7] Alaiya AA, Franzen B, Auer G, et al. Cancer proteomics:from identification of novel markers to creation of artifical learning models for tumor classification[J]. Electrophoresis,2000,21(6):1210-1217.
[8] Aicher L, Wahl D, Arce A, et al. New insights into cy-closporineA nephro toxicity by proteome analysis[J]. Electrophoresis, 1998, 19(11):1998-2003.
[9] Ramsamooj P, Notario V, Dritschilo A. Modification of nucleolar protein B23 after exposure to ionizing radiation[J]. Radiat Res, 1995, 143(2):158-164.
[10] Gamble SC, Dunn MJ, Wheeler CH, et al. Expression of proteins coincident with inducible radioprotection in human lung epithelial cells[J]. Cancer Res, 2000, 60(8):2146-2151.
[11] Gupta A, Andrews KL, Daniel KM, et al. Experimental induction of rhabdomyosarcoma in mice with fractionated doses of beta irradi-ation[J]. J Cancer Res Clin Oncol, 1999, 125(5):257-267.
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备注/Memo

备注/Memo:
收稿日期:2002-09-27。
作者简介:王治东(1977-),男,硕士研究生,主要从事辐射血液学研究。;陈肖华(1958-),男,副研究员,主要从事放射病实验治疗研究。
更新日期/Last Update: 1900-01-01