[1]李辉,孙俊杰,李卫鹏,等.99Tcm-MIBI脂质体的制备及特征分析[J].国际放射医学核医学杂志,2018,(5):425-429.[doi:10.3760/cma.j.issn.1673-4114.2018.05.007]
 Li Hui,Sun Junjie,Li Weipeng,et al.Preparation and characteristic analysis of 99Tcm-MIBI liposomes[J].International Journal of Radiation Medicine and Nuclear Medicine,2018,(5):425-429.[doi:10.3760/cma.j.issn.1673-4114.2018.05.007]
点击复制

99Tcm-MIBI脂质体的制备及特征分析(/HTML)
分享到:

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
期数:
2018年第5期
页码:
425-429
栏目:
基础研究
出版日期:
2018-10-01

文章信息/Info

Title:
Preparation and characteristic analysis of 99Tcm-MIBI liposomes
作者:
李辉1 孙俊杰2 李卫鹏1 訾刚1 袁超1
1. 233004, 蚌埠医学院第一附属医院核医学科;
2. 233030, 蚌埠医学院核医学教研室
Author(s):
Li Hui1 Sun Junjie2 Li Weipeng1 Zi Gang1 Yuan Chao1
1. Department of Nuclear Medicine, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China;
2. Department of Nuclear Medicine, Bengbu Medical College, Bengbu 233030, China
关键词:
脂质体99m锝甲氧基异丁基异腈同位素标记
Keywords:
Liposomes Technetium Tc 99m Sestamibi Isotope labeling
DOI:
10.3760/cma.j.issn.1673-4114.2018.05.007
摘要:
目的 研究99Tcm-甲氧基异丁基异腈(MIBI)脂质体的制备方法,并对其特征进行分析。方法 采用乙醇注入-超声法制备99Tcm-MIBI脂质体,测定脂质体粒径大小和包封率,对超声时间、大豆卵磷脂与胆固醇的质量比等条件进行优化,观察99Tcm-MIBI脂质体的稳定性。结果 当制备的99Tcm-MIBI质量浓度为0.01 mg/mL时,平均粒径大小为(171.4±25.2)nm,包封率的平均值为(8.5±1.3)%。大豆卵磷脂与胆固醇的质量比为4∶1和超声时间为5 min为最佳条件。电镜图显示,制备第1天后粒径较小,形态较为规则、均匀,呈完整球形或椭圆形;30 d后粒径大小及形态未见明显改变。结论 采用乙醇注入-超声法制备的99Tcm-MIBI脂质体粒径较小、包封率高,且较稳定。
Abstract:
Objective To study the preparation and analyze the characteristics of 99Tcm-methoxy isobutyl isonitrile (MIBI) liposomes. Methods 99Tcm-MIBI liposomes were prepared through the ethanol injection-ultrasonication method. Liposome size and encapsulation efficiency were determined, and ultrasonication duration and soybean lecithin-to-cholesterol mass ratio were optimized to observe their effects on the stability of 99Tcm-MIBI liposomes. Results When the mass concentration of 99Tcm-MIBI was 0.01 mg/mL, the average particle size was (171.4±25.2) nm, and the average encapsulation efficiency was (8.5±1.3)%. The optimal preparation conditions consisted of the mass ratio of soy lecithin to cholesterol of 4:1, the 99Tcm-MIBI mass concentration of 0.01 mg/mL, and ultrasonication duration of 5 min. Electron micrograph images revealed that after the first day, particles reduced in size and appeared regular and uniform in shape. The particles appeared completely spherical or elliptical. Particle sizes and shapes negligibly changed after 30 d. Conclusion 99Tcm-MIBI liposomes with small particle size, high encapsulation efficiency, and stable entrapment efficiency can be obtained through the ethanol-ultrasonication technique.

参考文献/References:

[1] Wilkins DJ, Ottewill RH, Bangham AD. On the flocculation of sheep leucocytes:I. Electrophoretic studies[J]. J Theor Biol, 1962, 2 (2):165-175. DOI:10.1016/0022-5193 (62)90045-0.
[2] Landesman-Milo D, Ramishetti S, Peer D. Nanomedicine as an emerging platform for metastatic lung cancer therapy[J]. Cancer Metastasis Rev, 2015, 34 (2):291-301. DOI:10.1007/s10555-015-9554-4.
[3] Alam MI, Paget T, Elkordy AA. Formulation and advantages of furazolidone in liposomal drug delivery systems[J]. Eur J Pharm Sci, 2016, 84:139-145. DOI:10.1016/j.ejps.2016.01.017.
[4] 赵新明, 韩静雅, 贾立镯, 等. 脂质体介导99Tcm-EGFR mRNA反义肽核酸在荷SKOV3卵巢癌裸鼠体内的分布及显像[J]. 中华核医学与分子影像杂志, 2014, 34 (5):379-384. DOI:10.3760/cma.j.issn.2095-2848.2014.05.010. Zhao XM, Han JY, Jia LZ, et al. Imaging and biodistribution of lipofectamine-mediated 99Tcm labeled EGFR mRNA antisense peptide nucleic acid in SKOV3 tumor-bearing nude mice[J]. Chin J Nucl Med Mol Imaging, 2014, 34 (5):379-384.
[5] Yin T, Wang P, Zheng R, et al. Nanobubbles for enhanced ultrasound imaging of tumors[J]. Int J Nanomedicine, 2012, 7:895-904. DOI:10.2147/IJN.S28830.
[6] 刘开春. 脂质体纳泡超声造影剂的制备及体外超声显像效果观察[J]. 医学影像学杂志, 2017, 27 (7):1375-1378. Liu KC. Preparation of gas-coated liposomesand evaluation of its enhancement effect for ultrasound imaging in vitro[J]. J Med Imaging, 2017, 27 (7):1375-1378.
[7] Homma T, Manabe O, Ichinokawa K, et al. Breast cancer detected as an incidental finding on 99Tcm-MIBI scintigraphy[J/OL]. Acta Radiol Open, 2017, 6 (7):2058460117715665[2018-01-31]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5533265. DOI:10.1177/2058460117715665.
[8] 李建勋, 齐德林, 盛权根, 等. 99mTc-MIBI显像对乳腺肿块鉴别诊断的价值[J]. 临床军医杂志, 2002, 30 (5):39-41. DOI:10.3969/j.issn.1671-3826.2002.05.016. Li JX, Qi DL, Sheng QG, et al. 99mTc-MIBI Scintigraphy in the Evaluation of the Breast Cancer Diagnosis[J]. Clin J Med Off, 2002, 30 (5):39-41.
[9] Gudlur S, Sandén C, Matou ková P, et al. Liposomes as nanoreactors for the photochemical synthesis of gold nanoparticles[J]. J Colloid Interface Sci, 2015, 456:206-209. DOI:10.1016/j.jcis.2015.06.033.
[10] Rodriguez-Evora M, Reyes R, Alvarez-Lorenzo C, et al. Bone regeneration induced by an in situ gel-forming poloxamine, bone morphogenetic protein-2 system[J]. J Biomed Nanotechnol, 2014, 10 (6):959-969. DOI:10.1166/jbn.2014.1801.
[11] 邓启盼. 纳米脂质体应用于肿瘤研究的进展[J]. 国际病理科学与临床杂志, 2010, 30 (6):489-494. DOI:10.3969/j.issn.1673-2588. 2010.06.008. Deng QP. Progress in the applications of nanoliposomes in cancer research[J]. J Int Pathol Clin Med, 2010, 30 (6):489-494.
[12] 涂宗财, 马达, 王辉, 等. 薄膜-探头式超声法和注乙醇-DHPM法制备的鱼油脂质体的理化性质研究[J]. 食品工业科技, 2015, 36 (14):127-130. DOI:10.13386/j.issn1002-0306.2015.14.017 Tu ZC, Ma D, Wang H, et al. Physicochemical properties of fish oil nanoliposomes made by thin-film rehydration-ultrasonic and ethanol injection-DHPM[J]. Sci Technol Food Ind, 2015, 36 (14):127-130.
[13] Belhaj-Tayeb H, Briane D, Vergote J, et al. In vitro and in vivo study of 99Tcm-MIBI encapsulated in PEG-liposomes:a promising radiotracer for tumour imaging[J]. Eur J Nucl Med Mol Imaging, 2003, 30 (4):502-509. DOI:10.1007/s00259-002-1038-4.
[14] 袁超, 张勇, 焦举, 等. 载雄激素受体三螺旋形成寡核苷酸PEG-PLGA纳米粒子的制备与应用[J]. 中山大学学报 (医学科学版),2008, 29 (5):602-605. DOI:10.3321/j.issn:1672-3554. 2008.05.022. Yuan C, Zhang Y, Jiao J, et al. Preparation and Primary Application of Androgen Receptor Triplex-forming Oligonucleotides Loaded PEG-PLGA Nanoparticles[J]. J Sun Yat-Sen Univ (Med Sci), 2008, 29 (5):602-605.
[15] Lee JH, Shao S, Cheng KT, et al. 99mTc-Labeled Porphyrin-Lipid Nanovesicles[J]. J Liposome Res, 2015, 25 (2):101-106. DOI:10.3109/08982104.2014.932379.
[16] 赵颖, 张琳, 王澈. 抗肿瘤脂质体的制备及其靶向性研究进展[J]. 中国生化药物杂志, 2017, 37 (1):311-314. DOI:10.3969/j.issn.1005-1678.2017.01.093 Zhao Y, Zhang L, Wang C. Advances in studies of anti-cancer liposomes with its preparation and targeting[J]. Chin J Biochem Pharm, 2017, 37 (1):311-314.

相似文献/References:

[1]杨雷,袁卫红,王家平,等.99Tcm-MIBI显像与钼靶X线对乳腺癌诊断的比较研究[J].国际放射医学核医学杂志,2016,40(2):111.[doi:10.3760/cma.j.issn.1673-4114.2016.02.005]
 Yang Lei,Yuan Weihong,Wang Jiaping,et al.Diagnostic value of technetium 99Tcm sestamibi and X-ray mammography in breast cancer: a comparison study[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(5):111.[doi:10.3760/cma.j.issn.1673-4114.2016.02.005]
[2]樊孝廉,舒毅,曾春平,等.99Tcm-MIBI门控心肌灌注显像在2型糖尿病无症状心肌缺血患者中的价值[J].国际放射医学核医学杂志,2016,40(3):171.[doi:10.3760/cma.j.issn.1673-4114.2016.03.002]
 Fan Xiaolian,Shu Yi,Zeng Chunping,et al.Significance of 99Tcm-MIBI gated myocardial perfusion imaging in patients with type 2 diabetes but without myocardial ischemia symptoms[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(5):171.[doi:10.3760/cma.j.issn.1673-4114.2016.03.002]
[3]陈伟君,孙达.99Tcm-MIBI显像在乳腺癌新辅助化疗中的应用价值[J].国际放射医学核医学杂志,2015,39(6):487.[doi:10.3760/cma.j.issn.1673-4114.2015.06.011]
 Chen Weijun,Sun Da.Clinical value of 99Tcm-MIBI imaging in neoadjuvant chemotherapy of breast cancer[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):487.[doi:10.3760/cma.j.issn.1673-4114.2015.06.011]
[4]成钊汀,朱小华.99Tcm-MIBI显像在甲状旁腺功能亢进症中的应用及进展[J].国际放射医学核医学杂志,2015,39(1):37.[doi:10.3760/cma.j.issn.1673-4114.2015.01.009]
 Cheng Zhaoting,Zhu Xiaohua.Application and progress of 99Tcm-MIBI scintieraphy in parathyroidism[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):37.[doi:10.3760/cma.j.issn.1673-4114.2015.01.009]
[5]夏晓天,张永学.影像学检查在乳腺癌的诊断及疗效评估中的应用价值[J].国际放射医学核医学杂志,2010,34(4):242.[doi:10.3760/cma.j.issn.1673-4114.2010.04.014]
 XIA Xiao-tian,ZHANG Yong-xue.The value of imaging examinations in diagnosis and curative effect evaluation of breast cancer[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(5):242.[doi:10.3760/cma.j.issn.1673-4114.2010.04.014]
[6]谭兴普,黄艳.99Tcm-MIBI亲肿瘤延迟断层显像对肺占位病灶的诊断价值[J].国际放射医学核医学杂志,2010,34(5):297.[doi:10.3760/cma.j.issn.1673-4114.2010.05.012]
 TAN Xing-pu,HUANG Yan.Diagnostic value of tumor positive delay tomogram imaging with 99Tcm-MIBI for pulmonary neoplasms[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(5):297.[doi:10.3760/cma.j.issn.1673-4114.2010.05.012]
[7]龙再颖,曲新艳,单英丽.99Tcm-MIBI显像对甲状腺结节良恶性鉴别诊断价值的研究[J].国际放射医学核医学杂志,2010,34(2):80.[doi:10.3760/cma.j.issn.1673-4114.2010.02.004]
 LONG Zai-ying,QU Xin-yan,SHAN Ying-li.The diagnostic value of 99Tcm-MIBI imaging to benign and malignant thyroid lesions[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(5):80.[doi:10.3760/cma.j.issn.1673-4114.2010.02.004]
[8]苏莉,黄诚刚,牛文强,等.25例血清PTH升高的甲状旁腺相关疾病患者的诊断[J].国际放射医学核医学杂志,2010,34(2):84.[doi:10.3760/cma.j.issn.1673-4114.2010.02.005]
 SU Li,HUANG Cheng-gang,NIU Wen-qiang,et al.Nuclear medicine diagnostic experience for 25 patients with parathyroid disease accompanied elevated serum PTH level[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(5):84.[doi:10.3760/cma.j.issn.1673-4114.2010.02.005]
[9]陈贵兵,黄劲雄,何小江,等.99Tcm-MIBI诊断重度淤胆型婴儿肝炎综合征的价值[J].国际放射医学核医学杂志,2010,34(3):168.
 CHEN Gui-bing,HUANG Jin-xiong,HE Xiao-jiang,et al.99Tcm-MIBI hepatobiliary scintigraphy in peadiatric patients with severe cholestatic infant hepatitis syndrome[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(5):168.
[10]胡旻,刘雅洁,许小飞,等.99Tcm-MIBI动态血流灌注显像和双时相核素显像对甲状腺冷结节良恶性的诊断价值研究[J].国际放射医学核医学杂志,2010,34(3):174.
 HU Min,LIU Ya-jie,XU Xiao-fei,et al.Study on the diagnostic value of 99Tcm-MIBI dynamic blood flow perfusion imaging and double-phase radionuclide imaging in benign and malignant cold thyroid nodules[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(5):174.

备注/Memo

备注/Memo:
收稿日期:2018-02-01。
基金项目:安徽省高等学校自然科学研究项目(KJ2015B071by)
通讯作者:袁超,Email:ychsysu@163.com
更新日期/Last Update: 2018-10-01