[1]何淑雅,蒋雨薇,王五洲,等.不同剂量137Cs γ射线照射对雌果蝇的辐射损伤和氧化效应[J].国际放射医学核医学杂志,2018,(2):143-147.[doi:10.3760/cma.j.issn.1673-4114.2018.02.008]
 He Shuya,Jiang Yuwei,Wang Wuzhou,et al.Irradiation damage and oxidative effects of different doses by 137Cs gamma ray on female Drosophila melanogaster[J].International Journal of Radiation Medicine and Nuclear Medicine,2018,(2):143-147.[doi:10.3760/cma.j.issn.1673-4114.2018.02.008]
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不同剂量137Cs γ射线照射对雌果蝇的辐射损伤和氧化效应(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
期数:
2018年第2期
页码:
143-147
栏目:
基础研究
出版日期:
2018-05-09

文章信息/Info

Title:
Irradiation damage and oxidative effects of different doses by 137Cs gamma ray on female Drosophila melanogaster
作者:
何淑雅12 蒋雨薇1 王五洲1 贺俊彦2 郭忠忠2 贺特1 肖方竹2 马云1
1. 421001 衡阳, 南华大学生物化学与分析生物学教研室;
2. 421001 衡阳, 南华大学公共卫生学院放射医学教研室
Author(s):
He Shuya12 Jiang Yuwei1 Wang Wuzhou1 He Junyan2 Guo Zhongzhong2 He Te1 Xiao Fangzhu2 Ma Yun1
1. Department of Biochemistry and Molecular Biology, University of South China, Hengyang 421001, China;
2. Department of Radiation Medicine, School of Public Health, University of South China, Hengyang 421001, China
关键词:
γ射线黑腹果蝇辐射损伤实验性氧化损伤
Keywords:
Gamma raysDrosophila melanogasterRadiation injuries experimentalOxidative injuries
DOI:
10.3760/cma.j.issn.1673-4114.2018.02.008
摘要:
目的 了解不同剂量γ射线照射对雌果蝇产生的辐射损伤与氧化效应。方法 采用不同剂量137Cs γ射线照射白眼W1118、红眼UAS-EGFP和Actin-Gal4雌果蝇,再测定其生存、运动和生殖能力。通过测定雌果蝇体内过氧化氢酶(CAT)活力和丙二醛(MDA)含量对果蝇成虫的辐射和氧化损伤进行评价。组间比较均采用LSD-t检验进行统计学分析。结果 137Cs γ射线对W1118、UAS-EGFP、Actin-Gal4的半数致死剂量分别为1513、1643、1809 Gy;3个品系3日龄雌果蝇成虫的生存率、攀爬高度分数、产卵量与γ射线照射剂量呈负相关;与未照射组相比,1550 Gy照射后雌果蝇成虫体内的CAT活力降低,差异有统计学意义(t=10.76、13.84、11.22,均P<0.05);MDA含量升高,差异有统计学意义(t=4.51、4.26、4.35,均P<0.05)。结论 高剂量γ射线照射导致的雌果蝇成虫的生存、运动和生殖能力降低可能与其氧化损伤相关。
Abstract:
Objective To investigate the radiation damage and oxidative effects of different doses by gamma radiation on female adult Drosophila. Methods Three strains of female Drosophila melanogaster of white eye W1118, red eye UAS-EGFP, and Actin-Gal4 were treated with different doses of 137Cs-gamma rays. Then, their survival, movement, and reproductive abilities were measured. The oxidative damage of the female adult Drodophila. melanogaster was evaluated by measuring catalase (CAT) activity and malondialdehyde (MDA) content. Results The half lethal doses of W1118, UAS-EGFP, and Actin-Gal4 were 1513, 1643, and 1809 Gy, respectively. The survival rate, climbing height fraction, and fecundity of the three-day-old female Drosophila were negatively correlated with the radiation dose. After irradiation, the activities of CAT decreased (t=10.76, 13.84, 11.22, all P<0.05) and the content of MDA increased (t=4.51, 4.26, 4.35, all P<0.05) in the female Drosophila adult body. Conclusion The reduced survival, movement, and fecundity of female adult Drosophila. melanogaster may be related to the oxidative damage under high doses of gamma radiation.

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备注/Memo

备注/Memo:
收稿日期:2017-11-16。
基金项目:国家自然科学基金(81741143);湖南省教育厅科研项目(17A186);湖南省研究生科研创新项目(CX2017B543)
通讯作者:马云,Email:luckymayun@163.com
更新日期/Last Update: 2018-05-09